Table 1 Comparative analysis of the accuracy and quality of sequences
From: Accuracy and quality assessment of 454 GS-FLX Titanium pyrosequencing
# of sequences | % of error-free sequences | # of positions | Insertions | Deletions | Mismatch | Ambiguous | Total % of error | |
|---|---|---|---|---|---|---|---|---|
GS20 (101) | 34015 | 82.00% | 32801429 | 0.18% | 0.13% | 0.08% | 0.10% | 0.49% |
Ref 1 (101) | 16052 | 87.12% | 1605640 | 0.15% | 0.05% | 0.01% | 0.01% | 0.22% |
Ref 2 (101) | 16466 | 60.01% | 1600327 | 0.42% | 0.23% | 0.04% | 0.01% | 0.70% |
Ref 3 (101) | 12215 | 72.96% | 1228804 | 0.17% | 0.19% | 0.01% | 0.01% | 0.38% |
Ref 4 (101) | 9908 | 56.43% | 984452 | 0.30% | 0.37% | 0.03% | 0.00% | 0.70% |
Ref 5 (101) | 15880 | 50.93% | 1595718 | 0.34% | 0.48% | 0.05% | 0.01% | 0.88% |
Ref 6 (101) | 15716 | 75.17% | 1581075 | 0.25% | 0.10% | 0.00% | 0.01% | 0.36% |
Total | 86237 | 67.57% | 8596016 | 0.27% | 0.23% | 0.02% | 0.01% | 0.53% |
Ref 1 (572) | 16052 | 6.75% | 5359696 | 0.52% | 0.46% | 0.10% | 0.12% | 1.20% |
Ref 2 (552) | 16466 | 9.75% | 4789285 | 0.89% | 0.28% | 0.10% | 0.08% | 1.35% |
Ref 3 (500) | 12215 | 18.75% | 4180478 | 0.30% | 0.35% | 0.07% | 0.12% | 0.84% |
Ref 4 (532) | 9908 | 6.88% | 2572843 | 0.56% | 0.71% | 0.19% | 0.11% | 1.57% |
Ref 5 (592) | 15880 | 7.46% | 6171098 | 0.38% | 0.38% | 0.06% | 0.07% | 0.89% |
Ref 6 (516) | 15716 | 11.81% | 6027338 | 0.60% | 0.17% | 0.07% | 0.04% | 0.88% |
Total | 86237 | 10.09% | 29100738 | 0.54% | 0.36% | 0.09% | 0.09% | 1.07% |