BAC-library1,2 | No. of clones | Average insert size (Kb) | Genome coverage | Cloning strategy | Vector | E. coli strain |
|---|
HVVMRXALLhB3 | 115,200 | 93 | 2.4 | Hin dIII, partial | pIndigo BAC5 | DH10B |
HVVMRXALLhC3 | 153,600 | 114 | 3.4 | Hin dIII, partial | pIndigo BAC5 | DH10B |
HVVMRXALLeA4 | 147,456 | 126 | 3.7 | Eco RI, partial | pIndigo BAC536 | DH10B |
HVVMRXALLmA5 | 202,752 | 143 | 6 | Mbo I, partial | pTAR BAC1.3 | DH10B |
HVVMRXALLrA6 | 253,440 | 92 | 4.7 | Mech. Sheared | pSMRT BAC | DH10B |
- 1Naming follows an agreed nomenclature for Triticeae BAC libraries: three letters species code (H ordeum v ulgare ss. v ulgare), three letters cultivar code (M or ex), three letters genome representation (i.e. ALL = whole genome, 1HS = short arm chromosome 1H), one letter enzyme (i.e. h = Hind III, e = EcoR I, m = Mbo I, r = Random/Mechanically sheared), one letter library identifier in case of multiple libraries per enzyme; 2all libraries are accessible via http://cnrgv.toulouse.inra.fr/en/library/barley; libraries were produced at: 3Australian Centre of Plant Functional Genomics, 4Clemson University Genomics Institute, 5Childrens Hospital Oakland, 6Lucigen corporation.
