Table 1 Assignment of the 1H NMR spectral signals of the apolar fraction.
Peak number | Chemical shift (ppm) | Assignmenta |
|---|---|---|
1 | 0.68 | Total cholesterol C18H3 |
2 | 0.85/0.86 | Total cholesterol C26H3/C27H3 |
3 | 0.86-0.91 | Acyl groups CH3(all except n-3) |
4 | 0.92 | Total cholesterol C21H3 |
5 | 0.98 | n-3 acyl groups CH3 |
6 | 1.01 | Free cholesterol C19H3 |
7 | 1.02 | Esterified cholesterol C19H3 |
8 | 1.06-1.19 | Multiple cholesterol protons |
9 | 1.20-1.38 | Acyl groups -(CH2)n- |
10 | 1.50-1.65 | Acyl groups -OCO-CH2-CH2- and H2O |
11 | 1.79-1.88 | Multiple cholesterol protons |
12 | 1.97-2.10 | Acyl groups -CH2-CH=CH- |
13 | 2.24-2.38 | Acyl groups -OCO-CH2- |
14 | 2.72-2.88 | Acyl groups =HC-CH2-CH= |
15 | 3.28-3.38 | Choline N(CH3)3 |
16 | 3.90-4.02 | Glycerophospholipids N-CH2 |
17 | 4.10-4.32 | Glycerol backbone -CH2OCOR |
18 | 4.33-4.43 | Phosphatidylcholine PO-CH2 |
19 | 4.50-4.66 | Esterified cholesterol C3H |
20 | 5.17-5.24 | Glycerophospholipid backbone >CH OCOR |
21 | 5.24-5.28 | Glycerol backbone >CH OCOR |
22 | 5.29-5.43 | Acyl groups -CH=CH- |