Selenium toxicity but not deficient or super-nutritional selenium status vastly alters the transcriptome in rodents

Table 2 Transcripts down regulated by Se deficiency

Genbank Accession	Gene Symbol	Gene Title	Fold Change¹	adj P-value²	Nrf2 Target³
Mouse Liver
NM_009156	Sepw1	selenoprotein W, muscle 1	-3.73	1.7E-05
NM_001033166	Selh	selenoprotein H	-2.83	0.0009
NM_008160	Gpx1	glutathione peroxidase 1	-2.14	0.0001
NM_019979	Selk	selenoprotein K	-2.14	0.0012
NM_013711	Txnrd2	thioredoxin reductase 2	-1.64	0.0091
			- - - - - - - - - - - - - - - - - - - - - - - - -
NM_015762	Txnrd1	thioredoxin reductase 1	-1.57	0.099	X
Mouse Kidney
NM_001033166	Selh	selenoprotein H	-2.64	0.0004
NM_009156	Sepw1	selenoprotein W, muscle 1	-2.64	0.0044
NM_008160	Gpx1	glutathione peroxidase 1	-2.46	0.0039
			- - - - - - - - - - - - - - - - - - - - - - - - -
Rat Liver ⁴
NM_001114939	Selh	selenoprotein H	-6.06	< 0.001
NM_030826	Gpx1	glutathione peroxidase 1	-3.73	< 0.001
NM_001014253	Selt	selenoprotein T	-1.60	0.005
NM_001106609	Txnrd3	thioredoxin reductase 3	-1.60	0.03
			- - - - - - - - - - - - - - - - - - - - - - - - -
NM_022584	Txnrd2	thioredoxin reductase 2	-1.57	0.06
NM_207589	Selk	selenoprotein K	-2.46	0.07

Transcripts that were significantly (above dashed line, P < 0.05) or nearly significantly (below dashed line, 0.05 < P < 0.2) down-regulated in Se-deficient rodent tissues compared to Se-adequate (0.2 or 0.24 μg Se/g diet, for mouse and rat studies respectively). ¹Fold changes determined by analysis of RMA expression data with the Limma package in R software, ²P-values were adjusted for multiple testing, ³Genes known to be Nrf2 targets, determined as described in the text, ⁴Genes down-regulated in Se-deficient liver from rats in study 2.

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