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Table 1 Mappable reads per lane in each of the three experiments.

From: RNA-seq: technical variability and sampling

Experiment

BR

TR

Mappable Reads

Exons detected

Exons with an average coverage of more than 5 reads per nucleotide

Contigs present in all samples of each experiment

c167

1

1

5888686

39156

13432

19248

c167

1

2

5951769

39202

13517

19248

c167

1

3

7146461

39954

15684

19248

c167

1

4

7544117

40201

16355

19248

c167

1

5

7377032

40120

16089

19248

D. sim.

1

1

5174398

45878

14517

20339

D. sim.

1

2

4979485

45808

13912

20339

D. sim.

2

1

27595266

51701

35303

20339

D. sim.

2

2

28691914

51857

35942

20339

D. sim.

3

1

27601233

51834

34968

20339

D. sim.

3

2

27748704

51822

35008

20339

D. mel.

2

1

10584341

48114

13396

17864

D. mel.

2

2

13399722

49073

19916

17864

D. mel.

3

1

12065885

48281

14794

17864

D. mel.

3

2

11794255

48319

17961

17864

D. mel.

4

1

10375138

47812

15718

17864

D. mel.

4

2

9283979

47460

14344

17864

  1. BR indicates a biological replicate and TR indicates a technical replicate. The experiments are described in Figure 1. There are a total number of 60,277 exons corresponding to distinct genomic regions in Flybase 5. In this replicate 14,972 replicates disagree by at least 1 log and 204 disagree by 2 or more logs.