Figure 3

KIR 454 Titanium Amplicon Primer Design. A) Schematic representation of macaque KIR3D and KIR2D molecules showing domain structure. B) Variability plot for a cDNA sequence alignment of all published KIR3DL alleles. PCR primer sites are indicated along with the region amplified by PCR. Note that 454 sequencing reads span the D1, D2, and stem (ST) regions. The signal sequence (SS), D0, transmembrane region (T), and cytoplasmic region (C) are not amplified by our PCR primers.