Figure 3
Two-dimensional SDS-Page map of immunized Galleria larvae. Hemolymph protein from untreated and LPS-immunized larvae was loaded on 24-cm pH 3 to 11 NL isoelectric focusing strips, followed by Tris-Tricine-SDS-polyacrylamide gel electrophoresis on a 15% gel. Image analysis enabled visualization of new or enhanced spots present in hemolymph samples from immunized larvae depicted in orange color. Putative identifications of immune-inducible proteins by MALDI-TOF analysis and according to our recent study [21] are depicted next to the respective spots. Molecular mass standards are indicated in kDa (left), and the pI range by an arrow. PGRP, peptidoglycan recognition protein; GNBP, Gram negative bacteria binding protein; GST, Glutathione-S-transferase; Apo III, apolipophorin III; DLS..., unknown protein.