Figure 1

Features of small RNAs mapped to an IAP consensus sequence (A) The lengths of small RNAs that mapped to IAP are presented. Reads mapped to the sense strand are presented above the x-axis, and reads mapped in antisense below. (B) The sequence composition of the IAP-derived 19mers and piRNAs were obtained from sequence logos. The reads were separated by their orientation relative to an IAP reference sequence and plotted separately, as indicated. The sequence logos were also extended beyond the 3' ends of the reads (unboxed regions) to reveal any downstream sequence bias. (C) The density of 19mer RNAs mapped to IAP in sense (only reads with an A 10nt downstream and absence of U immediately downstream of the 3' terminus were used), secondary piRNAs mapped to IAP in sense (24-30nt RNAs with A in position 10 and absence of 5' U) and primary-piRNAs mapped to IAP in antisense (24-30nt RNA with 5' terminal U and absence of A in position 10), are plotted. Only the 5' ends of the reads were used to calculate the density. (D) The abundances of IAP-derived p-piRNAs and s-piRNAs, at varying distances from IAP-derived piRNA-related 19mers (pr19RNAs) 5' ends, were tallied and are presented as proportions of the total RNA count.