Figure 2

Evaluation of plant and fungal contents in infected lentil leaflet tissues, and cytological analysis of an infection time-course. (A) Semiquantitative RT-PCR amplification of the C. truncatum and L. culinaris 60S ribosomal transcripts in the appressorial penetration phase (16 hai), biotrophic phase (44 hai) and necrotrophic phase (68 hai). Fungal 60S transcripts were also qualitatively assessed in control mixtures, which were obtained by combining 0, 10, 20, 30, 40, 50, and 100% RNA from fungal mycelium into RNA from mock-inoculated leaflets. Twenty six cycles were used for amplification. (B) In planta infection time- course. C. truncatum infected lentil leaflets cultivar 'Eston' at 42-44 hai represent the biotrophic phase characterized by large intracellular primary hyphae and the necrotrophic phase at 68 hai. *Appressorium, IP, Infection peg. Bars = 10 μm.