Figure 4

Distribution and evolutionary conservation of TFBSs in promoter regions of DC-expressed miRNAs. (A) The TFBS motif scores (in blue, y-axis range from 0 to 15 per subgraph) as calculated using Clover and the extent of evolutionary conservation as PhastCons score (in grey, y-axis on the right). (B) Distribution pattern of TFBSs, selected based on various motif score and evolutionary score thresholds, in the promoter region of miRNAs up-regulated in DCs. (C) Comparison of the median conservation scores of the bases in the predicted TFBSs in the promoters of miRNAs that are over-expressed in DCs, with those of randomly chosen sequences of the same length from the same promoters. Boxplots represent the median and interquartile range of the median PhastCons conservation scores. The fold shown is the ratio of the median of the DC conservation scores and the median of the conservation scores of the random sets (n = number of TFBS instances concerned).