Figure 4

Overview on new CDS identified via microarray and RT-PCR analysis. (A) Transcriptional patterns of the new CDS based on microarry analysis. Constitutive expression indicates that these CDS are constitutively expressed at OD₆₀₀ = 1.0, 1.6 and 2.0 during the growth. DSF-regulated expression indicates that the expression levels of these CDS at OD₆₀₀ = 2.0 are significantly lower in ΔrpfF, ΔrpfC, or Δclp strains than those in wild type. NYG-induced expression indicates that this CDS is only transcribed at OD₆₀₀ = 2.0 when grown in NYG medium. (B) RT-PCR analysis to verify the expression difference between the wild type and the rpfF deletion mutant ΔrpfF. The 4 new CDS supported by extrinsic evidence were indicated by bold font.