Figure 3

Orthologs of the L. kluyveri gene SAKL0B06622g discovered in eight species. (A) Output for web SearchDOGS with SAKL0B06622g used as a query. (i) The dashed box highlights the genomic segment containing the query. (ii) The letter N indicates the hits to noncoding regions in other species; C1 and C2 indicate coding regions. (iii) S6 and S7 refer to segments of the Ancestral genome. Genomic segments in other species that map to the same Ancestral segments constitute an OGS group. Six noncoding hits map to the same ancestral region (S6) as the query (dashed red boxes) and the seventh, located between YBL027W and YBL026W in S. cerevisiae, can be mapped to an adjacent ancestral region. (iv) 'UNDEF3' for the S. bayanus and S. cerevisiae genomic segments indicates that they have undergone some rearrangement relative to the Ancestor. The Ancestral segments to which they map are listed as "singlehit" if they are not shared with any other species. (B) YGOB screenshot after addition of the new genes, which are indicated by the dashed box. An ortholog of this gene is also inferred to have existed in the Ancestral genome because it is present in both non-WGD and post-WGD species. (C) ClustalW multiple sequence alignment [45] of the inferred protein sequences from nine species. (D) Location of the newly inferred S. cerevisiae gene YBL026W-A (green arrow), superimposed on a screenshot of the relevant region of chromosome II from SGD [46]. Eight expressed sequence tags from Miura et al. [16] indicate transcription of the gene.