Figure 2

Phenotypes observed after the injection of dsRNA for select GPCR into T. castaneum. dsRNA for malE (control) and selected T. castaneum GPCR were injected into one-day old final instar larvae. Phenotypes observed in control insects at three days after ecdysis to the final instar larval stage (A); quiescent stage, a non-feeding prepupal stage (B); three days after ecdysis to the pupal stage (C); three days after adult eclosion (D) are shown. (E). Phenotypes of TC007490/D2R RNAi insects. (F). Phenotypes of TC001872/Cirl RNAi insects. Accumulation of the ommatidia (black arrow) at quiescent stage is shown at the upper-left panel at higher magnification. (G). Phenotypes of TC012521/stan RNAi insects with wings attached to the ventral side of the abdomen (black arrow). (H). Phenotypes of TC014055/fz RNAi insects. The black arrow points to the split in the dorsal thoracic region. (I). Phenotypes of TC005545/smo RNAi insects with unextended pupal wings (white arrow). (J). Phenotypes of TC009370/mthl RNAi insects with improperly folded wings (white arrow) and unshed exuviae (white arrow head). Scale bar: 1.0 mm.