Table 4 Evaluation of SNP primer pairs in four carrot genetic stocks used to develop the EST library
PCR results | Number of primers | Percentage of all primer tested | Percentage of amplified primer | Percentage of amplified and sequenced primers |
|---|---|---|---|---|
SNP | Â | Â | Â | Â |
Tested | 354 | - | - | - |
Amplified | 311 | 88 | - | - |
Single product | 272 | 77 | 87 | - |
   Single expected product | 162 | 46 | 52 | - |
   Single larger product | 110 | 31 | 35 | - |
Multiple product | 39 | 11 | 13 | - |
Sequenced* | 258 | 73 | 83 | - |
Polymorphic | 212 | 60 | 68 | 82 |