Table 1 S. cerevisiae mutants with increased levels of dually phosphorylated MAP Kinase Slt2

YAL058W

CNE1

16.4

Cell wall and morphogenesis

Calnexin 1. may be involved in a QC process for secretory pathway proteins

YBL007C

SLA1

8.1

Cell wall and morphogenesis

Functions in the assembly of cortical actin cytoskeleton

YBR078W

ECM33

14.4

• Cell wall and morphogenesis

GPI-anchored protein required for CW integrity and mannoprotein biosynthesis

YCR088W

ABP1

2.3

Cell wall and morphogenesis

Actin binding protein that functions in clathrin- and actin-mediated endocytosis

YDL095W

PMT1

10.8

Cell wall and morphogenesis

Mannosyltransferase, first step in O-glycosylation

YDR349C

YPS7

16.6

• Cell wall and morphogenesis

GPI-anchored aspartyl protease involved in maintaining cell wall integrity

YJL062W

LAS21

3.6

Cell wall and morphogenesis

Protein required for addition of a side chain to the GPI core structure

YJL099W

CHS6

2.9

Cell wall and morphogenesis

Protein involved in chitin synthase Chs3p activity

YJR075W

HOC1

2.1

• Cell wall and morphogenesis

Alpha-1,6-mannosyltransferase activity

YLR319C

BUD6

2.4

Cell wall and morphogenesis

Budding 6, required for bipolar budding and involved in bud site selection

YLR337C

VRP1

8.6

Cell wall and morphogenesis

Proline-rich protein verprolin, involved in cytoskeletal organization

YLR350W

ORM2

2.6

Cell wall and morphogenesis

Putative transmembrane protein that may function in the CWI pathway

YLR370C

ARC18

5.5

Cell wall and morphogenesis

Component of the ARP2/3 actin-organizing complex, involved in actin assembly

YMR307W

GAS1

27.4

• Cell wall and morphogenesis

GPI-anchored protein with 1,3-beta-glucanosyltransferase activity

YNL079C

TPM1

7.2

• Cell wall and morphogenesis

Tropomyosin, functions in a variety of processes involving the actin cytoskeleton

YNL116W

DMA2

3.4

Cell wall and morphogenesis

Functions in spindle positioning and septin ring assembly

YNL192W

CHS1

2.0

Cell wall and morphogenesis

Chitin synthase I, has a repair function during cell separation

YOR002W

ALG6

6.3

Cell wall and morphogenesis

Dolichyl-phosphate-mannose-protein mannosyltransferase activity

YER037W

PHM8

4.0

Metabolism

Lysophosphatidic acid phosphatase

YJL137C

GLG2

2.3

Metabolism

Transferase activity, transferring hexosyl groups

YHL023C

RMD11

2.9

Nuclear

Protein possibly involved in meiotic nuclear division

YPR031W

NTO1

2.0

Nuclear

May be involved in chromatin-mediated transcription regulation

YBR101C

FES1

2.8

Protein metabolism

NEF for the cytosolic chaperone Ssa1p. Response to oxidative stress

YDR069C

DOA4

2.6

Protein metabolism

Ubiquitin-specific protease that acts in recycling ubiquitin

YFL007W

BLM10

4.0

Protein metabolism

Proteasome assembly

YBL024W

NCL1

5.8

RNA metabolism

tRNA (cytosine-5-)-methyltransferase activity

YPL029W

SUV3

2.0

RNA metabolism

Mitochondrial RNA helicase of the DEAD box family

YPL213W

LEA1

9.0

RNA metabolism

RNA splicing factor activity

YDL047W

SIT4

4.3

Signal transduction

Serine/threonine phosphatase involved in cell cycle regulation/ion homeostasis

YDR162C

NBP2

16.9

Signal transduction

Negative regulator of the HOG pathway

YDR389W

SAC7

7.5

Signal transduction

GTPase-activating protein for Rho1p

YER155C

BEM2

2.4

• Signal transduction

GTPase-activating (GAP) protein that regulates Rho1p

YHR082C

KSP1

2.9

Signal transduction

Serine/threonine kinase involved in filamentous growth

YHR206W

SKN7

2.5

Signal transduction

Transcription factor involved in the oxidative and osmotic stress responses

YJR074W

MOG1

5.4

Signal transduction

Involved in nuclear protein import, plays a role in osmoregulation via SLN1-SKN7

YKL126W

YPK1

2.3

Signal transduction

Putative S/T protein kinase possibly related with the CWI and sphingolipids

YLR371W

ROM2

2.5

Signal transduction

GDP-GTP exchange factor for Rho1p

YNL053W

MSG5

5.4

Signal transduction

Dual-specificity protein tyrosine phosphatase involved in response to pheromone

YNR047W

FPK1

3.9

Signal transduction

Serine/threonine protein kinase that regulates phospholipid asymmetry

YLR242C

ARV1

8.6

Sphingolipid metabolism

Protein involved in sterol uptake required for normal sphingolipid metabolism

YER139C

RTR1

5.2

Transcription

Protein required for growth at high temperature, RNA polymerase II factor

YMR136W

GAT2

10.7

Transcription

GATA zinc finger transcription factor

YNL025C

SSN8

2.2

Transcription

RNA polymerase II transcription mediator

YPL042C

SSN3

3.9

Transcription

Cyclin-dependent serine/threonine protein kinase of the RNA polymerase II

YPR065W

ROX1

4.7

Transcription

Transcriptional repressor of hypoxic genes

YPR115W

3.6

Transcription

Phosphoinositide binding. Response to oxidative stress

YDR200C

VPS64

3.9

Transport

Class B vacuolar sorting protein involved in Prc1p trafficking/α-factor secretion

YFL048C

EMP47

4.1

Transport

Golgi and ER membrane protein involved in glycoprotein secretion

YGR028W

MSP1

2.1

Transport

Intra-mitochondrial sorting protein, member of the AAA family of ATPases

YHR032W

ERC1

3.5

Transport

Member of the MatE family. Transporter activity

YJL133W

MRS3

5.2

Transport

Mitochondrial carrier (MCF, family of membrane transporters). Iron transport

YJR152W

DAL5

5.7

Transport

Member of the allantoate family of the major facilitator superfamily (MFS)

YLR292C

SEC72

2.9

Transport

Component of ER protein-translocation subcomplex.

YCR090C

2.0

Unknown

Unknown function

YDL173W

4.1

Unknown

Unknown function

YDR290W

4.2

Unknown

Unknown function

YGR022C

3.9

Unknown

Unknown function

YLR250W

SSP120

5.2

Unknown

Unknown function

YLR338W

7.2

Unknown

Unknown function

YMR119W-A

3.2

Unknown

Unknown function

YNL058C

3.0

Unknown

Unknown function

YNL105W

5.5

Unknown

Unknown function

YNR014W

2.5

Unknown

Unknown function

YPL158C

3.0

Unknown

Unknown function