A genomic biomarker signature can predict skin sensitizers using a cell-based in vitro alternative to animal tests

Table 3 List of reference chemicals used in assay development

Compound	Abbreviation	Potency	LLNA	HMT¹	HPTA¹
*Sensitizers*
2,4-Dinitrochlorobenzene	DNCB	Extreme [15]	+ [15]
Oxazolone	OXA	Extreme [15]	+ [15]
Potassium dichromate	PD	Extreme [14]	+ [14]	+	+
Kathon CG (MC/MCI)	KCG	Extreme [14, 45]	+ [14, 46]
Formaldehyde	FA	Strong [15]	+ [15]	+	+
2-Aminophenol	2AP	Strong [46]	+ [47]
2-nitro-1,4-Phenylendiamine	NPDA	Strong [46]	+ [47]
p-Phenylendiamine	PPD	Strong [47]	+ [48]	+	+
Hexylcinnamic aldehyde	HCA	Moderate [15]	+ [15]
2-Hydroxyethyl acrylate	2HA	Moderate [46]	+ [47]		+
2-Mercaptobenzothiazole	MBT	Moderate [46]	+ [47]	+	+
Glyoxal	GO	Moderate [46]	+ [47]	+
Cinnamaldehyde	CALD	Moderate [47]	+ [48]	+	+
Isoeugenol	IEU	Moderate [47]	+ [48]		+
Ethylendiamine	EDA	Moderate [14]	+ [14]
Resorcinol	RC	Moderate [48]	+ [49]	-	+
Cinnamic alcohol	CALC	Weak [46]	+ [48]
Eugenol	EU	Weak [47]	+ [48]		+
Penicillin G	PEN G	Weak [47]	+ [48]	+
Geraniol	GER	Weak [14]	+ [14]	-	+
*Non-sensitizers*
1-Butanol	BUT		- [50]
4-Aminobenzoic acid	PABA		- [51]	-	+
Benzaldehyde	BA		- [52]
Chlorobenzene	CB		- [14]
Diethyl phthalate	DP		- [48]
Dimethyl formamide	DF		- [46]
Ethyl vanillin	EV		- [52]
Glycerol	GLY		- [48]
Isopropanol	IP		- [48]
Lactic acid	LA		- [14]
Methyl salicylate	MS		- [14]	-
Octanoic acid	OA		- [53]
Propylene glycol	PG		- [51]
Phenol	PHE		- [53]	-
p-Hydroxybenzoic acid	HBA		- [54]
Potassium permanganate	PP		-
Salicylic acid	SA		- [14]	-
Sodium dodecyl sulphate	SDS		+² [14, 53]	-
Tween 80	T80		- [20]		+
Zinc sulphate	ZS		+² [55]

List of sensitizers and non-sensitizers used in assay development. 1) HMT, Human Maximization Test; HPTA, Human Patch Test Allergen. Information is derived from [17]. 2) False positives in LLNA.

ISSN: 1471-2164