Figure 2

Consequences of the lack of PKA in the induction by glucose of some representative genes. Yeast strains TPK1 TPK2 TPK3, tpk1 tpk2 tpk3 msn2 msn4, and tpk1 tpk2 tpk3 yak1 were grown on ethanol and samples were taken before the addition of glucose to the medium and 30 min after, as described in Methods. mRNA levels were measured using Affymetrix microarrays. Biological duplicates were performed. Expression data from biological replicates generally differed from the average by less than 25%. The induction factor is the quotient of the values measured in samples incubated in the presence of glucose and in those grown in the absence of glucose. Induction is considered "total" when the induction factor is no less than 70% of that measured in the reference strain in at least one of the strains lacking PKA.