Figure 7
Post-translational chemical medication (PTCM) analysis using de novo -Ustag approach. An example illustrating the application of de novo-UStag approach in detection of multiple modification and high resolution MS/MS for distinction between multiple possible combinations. C-terminus amidation and tryptophan oxidation to oxolactone was determined for the sequence SLKELVESDQKWR of the Salmonella thiamin/thiamin pyrophosphate ABC transporter protein. Explanation for the determination of modifications is detailed in text.