Figure 6

Hierarchical cluster analysis of adipogenic gene expression profile. Hierarchical cluster analysis of the 184 candidate genes (85 with increased expression, 99 with decreased expression) selected as relevant by SiPaGene. Clustering of genes and experiments resulted in two main groups, namely undifferentiated MSC at day 0 and all other experiments. The latter main group was subdivided in the native fat tissue group and the group of adipogenic induced MSC at day 1, 3, 7 and 17. The MSC group was further divided in two groups: in the first, we found all MSC cultures induced for 1 and 3 days (early adipogenesis) and 2 out of 3 MSC cultures induced for 7 days. In the second subgroup, we observed all MSC cultures stimulated for 17 days (later adipogenesis) and the third day 7 culture (see also the dendrogram). Colours represent relative levels of gene expression: bright red indicates the highest level of expression, and bright green indicates the lowest level of expression. On the right site gene names are included.