Table 3 Real-time PCR validation of selected genes identified by microarray analysis as differentially regulated
Gene (predicted function of encoded protein) | Fold-Change | |
|---|---|---|
| Â | Microarraya | Real-Time PCRb |
Genes differentially regulated in RSF34 compared to K56-2 | Â | Â |
BCAL0114 (Flagellin) | -5.8 | No change |
BCAL3490 (Exported protein) | 12.0 | 6.8 |
BCAM0083 (Hypothetical protein) | 12.1 | 10.0 |
BCAM0186 (Lectin) | 14.4 | 9.8 |
BCAM0537 (Serine peptidase) | 51.0 | 8.0 |
BCAM0855 (UDP-glucose dehydrogenase) | 22.2 | 13.5 |
BCAM1010 (putative UTP-glucose-1-phosphate) | 15.2 | 20.7 |
BCAM2453 (Redoxin) | 40.5 | 6.2 |
Genes differentially regulated in RSF34 4000B compared to RSF34 | Â | Â |
BCAL1083 (Exported alkaline phosphatase) | -16.7 | -3.2 |
BCAL1213 (2-oxoisovalerate dehydrogenase β subunit) | 29.9 | 19.7 |
BCAL1270 (Phosphate transport, periplasmic) | -23.5 | -17.9 |
BCAM0537 (Serine peptidase) | -28.2 | -4.1 |
BCAM0855 (UDP-glucose dehydrogenase) | -5.0 | -4.2 |
BCAM2195 (AMP-binding enzyme) | 22.8 | 4.9 |
BCAM2453 (Redoxin) | -22.5 | -1.9 |
Genes differentially regulated in K56-2 upon polymyxin B treatment | Â | Â |
BCAL0114 (Flagellin, FliC) | -4.9 | -10.3 |
BCAL0140 (Flagellar biosynthesis) | -25.9 | -26.2 |
BCAL0520 (Flagellar hook-length control, FlhB) | -15.1 | -131.9 |
BCAL0566 (Flagellar basal body rod modification, FlgD) | -17.1 | -7.7 |
BCAL1351 (Exported Protein) | 3.6 | 31.3 |
BCAL3507 (FliL) | -13.6 | -4.3 |
BCAM0083 (Hypothetical protein) | 21.4 | 34.5 |
BCAM2187 (Macrolide-specific ABC-type efflux) | 9.5 | 33.7 |