Figure 1

Fold-change of 16 miRs modulated in IFN-β-treated Huh-7 cells and in 21-5 replicon cells. Total RNA from both cell lines was used to assess miR levels using real time RT-PCR. miR levels from Huh-7 cells were set as the basis for the comparative results. miRs with a fold-change > 1.2 or < -1.2 were considered differentially expressed in 21-5 vs. Huh-7 cells. The mean of three independent biological replicas ± SD is reported; the RNA extract from each biological replica had been assayed by real-time RT-PCR in triplicate (three technical replicas per each RNA extract). (A) Concordant modulation (up- or down-regulation) of 9 miRs (miR-1, miR-30a-3p, miR-142-5p, miR-142-3p, miR-146a, miR-146b, miR-155, miR-128a and miR-296) in IFN-β-treated Huh-7 cells and in 21-5 replicon cells. (B) Modulation of five miRs (miR-196b, miR-125a, miR-122a, miR-30c and miR-30b) in 21-5 replicon cells. (C) Discordant modulation of two miRs (miR-128b and miR-196a) in IFN-β-treated Huh-7 cells and in 21-5 replicon cells.