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Figure 6 | BMC Genomics

Figure 6

From: Transcriptome profiling of the rice blast fungus during invasive plant infection and in vitro stresses

Figure 6

Gene profiling during a barley infection time-course. Eight genes were chosen from the microarray validation experiments with increased gene expression among all seven conditions (MAS3: MGG_09875.6; SOD: MGG_07697.6; xylanase: MGG_07868.6) and decreased gene expression among all seven conditions (cutinase: MGG_02393.6; endothiapepsin: MGG_02201.6; HSP30: MGG_05719.6; urea active transporter: MGG_09063.6; glutamine synthetase: MGG_06888.6), compared to the reference sample. Real-time qRT-PCR was performed on all eight genes, with glyceraldehyde 3-phosphate dehydrogenase (GAPDH) used as the endogenous control (housekeeping gene). Five time-points were examined, 24, 48, 72, 96 and 120 hours post-inoculation (hpi), and all samples were performed in triplicate.

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