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Table 9 Comparison of Fold-change values from qPCR target genes and microarray in the 3 day sampling.

From: Skin healing and scale regeneration in fed and unfed sea bream, Sparus auratus

  

WS

ST

STWS

ST vs STWS

SAPD ID

Target Transcript

qPCR

Probe 1

Probe 2

qPCR

Probe 1

Probe 2

qPCR

Probe 1

Probe 2

qPCR

Probe 1

Probe 2

SAPD03530

Collagen V alpha2

1.087*

0.780

0.758

0.836

0.814

0.782

0.646

0.598

0.580

0.772

0.734

0.741

SAPD01318

Collagen I alpha1

0.838

0.398

0.547

1.294*

0.570

0.770

0.467

0.281

0.413

0.361

0.492

0.536

SAPD26722

SPP1

0.552

0.116

0.130

1.109*

0.415

0.477

0.501

0.293

0.323

0.451

0.708

0.678

SAPD00344

p22phox protein

0.574

0.255

0.250

0.367

0.296

0.303

0.380

0.231

0.231

1.035*

0.780

0.763

SAPD13946

Retinol-binding protein I

3.097

1.738

2.117

0.877

0.680

0.765

1.840

2.023

2.329

2.098

2.976

3.044

SAPD02730

Glutathione S-transferase A5

1.846

1.774

1.726

0.697

0.873

0.900

2.168

2.080

1.896

3.112

2.384

2.017

SAPD23304

Cytochrome c

1.373

1.874

1.949

0.625

0.873

0.845

2.149

2.180

2.278

3.438

2.498

2.696

SAPD20351

Beta-2-glycoprotein I

4.507

2.292

2.698

6.490

9.503

10.862

3.492

3.870

4.304

0.538

0.407

0.396

SAPD05261

IFI56

2.144

3.291

3.213

0.372

0.422

0.417

1.385

2.193

1.906

3.728

5.202

4.574

SAPD02155

pCNA

1.329

1.754

1.62

0.558

0.517

0.489

1.598

1.692

1.585

2.862

3.273

3.240

  1. Comparisons were made for each treatment in relation to the control with the additional comparison of fasted vs. fasted without scales (ST vs. STWS). Values correspond to the Fold-change which was calculated by the ratio of mean expression values between treatment and control groups (individual biological replicates of n = 5 for both qPCR and microarray). * indicate where differences between qPCR and microarray data were observed.
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BMC Genomics

ISSN: 1471-2164

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