Table 1 Characteristics of conserved miRNA families and stem-loops in sexual Boechera genotypes.

miR156

UGACAGAAGAGAGA GAGCAC

Ath, U/A

5'

54.67

28.80

0.635

ET5PU7E02HBOCM

FR869734

miR157

UUGACAGAAGAGAGA GAGCAC

Sbi, U/A

5'

54.67

28.80

0.635

ET5PU7E02HBOCM

FR869734

miR159

UUUGGU UUGAAGGA AGCUCUA

Ath, A/U, G/A

-

-

-

-

ETM6Q5C04I3XE2^a

FR869757

miR160

UGCCUGGCUCCCUGUAUGCCA

Ath

-

-

-

-

ET5PU7E02JH8DI^a

FR869730

miR161

UGAAAGUGACUACAUCGGGGU

Ath

5'

55.43

24.67

0.554

ET5PU7E02IM9YA

FR869722

miR164

UGGAGAAGCAGGGCACGUAAA

Gar

-

-

-

-

ETM6Q5C04IAM5V^a

FR869752

miR166

CCGGACCAGGCUUCAUCCCAG

Pta

-

-

-

-

ET5PU7E02JKLSI^a

FR869725

miR167

UGAAGCUGCCAGCAUGAUCUA

Ath

5'

60.00

48.20

1.201

ETM6Q5C03GWWM2

FR869745

miR170

UGAUUGAGCCGCGCCAAUAUC

Ath

-

-

-

-

ETM6Q5C03GVN0G^a

FR869746

miR172

AGAAUCC UGAUGAUGCUGCAU

Ath, U/C

-

-

-

-

ET5PU7E02GZHSB^a

FR869721

miR319

UUGGAA UGAAGGGAGCUCCAC

Ath, A/C, U/A, U/C

-

-

-

-

ETM6Q5C03FYJ8Y^a

FR869740

miR394

UUGGCAUUCUGUCCACCUCC

Ath

5'

57.76

46.46

1.100

ET5PU7E02IYKJ5

FR869733

miR395

AUGAAGA GUUUGGAGGAACUC

Osa, U/A

-

-

-

-

ETM6Q5C03FTE98^a

FR869741

miR396

UCCACAGGCUUUCUUGAACGG

Ghr

5'

41.96

38.37

0.661

ET5PU7E02GM4DS

FR869731

miR398

UGUGA UCUCAGGUA ACCCCUU

Ath, U/A, C/A

-

-

-

-

ETM6Q5C04IDRCB^a

FR869755

miR399

UGCCAAAGGAGAUA UGCCCUA

Ath, U/A, G/A

-

-

-

-

ET5PU7E02FZ073^a

FR869726

miR400

UAUGAGAGUAUUAUAU GUCAC

Ath, A/U

3'

60.53

15.79

0.400

ET5PU7E02JJRIA

FR869735

miR403

UUAGAUUCACGCACAAACUCC

Ath, G/C

5'

57.33

24.93

0.584

ET5PU7E02I3RXE

FR869723

miR408

AUGCACUGCCUCUUCCCUGGC

Ath

3'

58.78

33.58

0.815

ETM6Q5C04JX15C

FR869749

miR414

UCAUCA UCAUCAUCAUCGUCG

UCAUCA UCAUCAUCAUCGUCA

UCAUCA UCAUCAUCAUCGUCA

Ath, U/A, A/G

Ath, U/A

Ath, U/A

5'

3'

3'

51.18

51.01

56.65

29.29

23.62

24.64

0.600

0.482

0.568

ET5PU7E02IZWR4

ET5PU7E02GNU3F

ET5PU7E02I14IE

FR869738

FR869727

FR869724

miR415

G ACAGAGA AGAAACAGAACAU

Ath, A/G, C/A

-

-

-

-

ETM6Q5C03FIL8C^a

FR869748

miR444

UUGCUGCCUCAAGCUC CCG GC

Zma, U/C, U/G

-

-

-

-

ETM6Q5C04IXD3L^a

FR869750

miR482

UCUUCCCUACACCG CCCAUAC

Gso, U/G

-

-

-

-

ET5PU7E02HNZGI^a

FR869720

miR529

G CUC UU CCCUCUCUCUUCUUC

Osa, C/G, G/C, A/U

-

-

-

-

ET5PU7E02HC551^a

FR869729

miR824

UAGACCAUUUGUGAGAAGA GA

Ath, G/A

-

-

-

-

ETM6Q5C04ISA8K^a

FR869754

miR835

UUU UUC CAUAUGUUCUUUAUC

Ath, C/U, G/C

-

-

-

-

ETM6Q5C04JNEVJ^a

FR869751

miR838

UUUUCUUCUACUUCUUC CC CA

Ath, G/C, A/C

-

-

-

-

ETM6Q5C03FOEE6^a

FR869747

miR841

UACGAC CCACUG GAAACUGAA

Ath, G/C, U/G

-

-

-

-

ETM6Q5C03HCEIS^a

FR869742

miR845

UAGCUCUGAUACCAAA UGAUA

Vvi, U/A

-

-

-

-

ET5PU7E02F58WO^a

FR869732

miR846

UUGAAUUGG AGUGCUUGC AUU

Ath, A/G, A/C

-

-

-

-

ETM6Q5C03FVWRY^a

FR869743

miR852

AAGAUAAGCGCCUUAGG UCUG

Ath, U/G

5'

62.92

38.31

1.033

ETM6Q5C03G2GJO

FR869744

miR854

GAUGAGGAG AA GGAGGAGGAG

Ath, U/G, G/A

-

-

-

-

ETM6Q5C04JC8OP^a

FR869756

miR859

UCUCUCUGUUGUGAAA UCAAA

Ath, G/A

-

-

-

-

ET5PU7E02GYEM5^a

FR869736

miR860

UCAG UAGC UUGGACUAUGUAU

Ath, A/G, A/C

-

-

-

-

ETM6Q5C03G8ZLD^a

FR869739

miR861

CCUUGGAGAAAUAUGCU UCAA

Ath, G/U

-

-

-

-

ET5PU7E02IMVAL^a

FR869728

miR865

UUUC UCCUCAAAUUUC UCCAA

Ath, U/C, A/C

-

-

-

-

ETM6Q5C04JQMWD^a

FR869753

miR869

CAUGGUUCAAUGCA GGUGC UA

Gma, U/A, U/C

-

-

-

-

ET5PU7E02JV51F^a

FR869737