Figure 4

Viability phenotypes of deo^- and thyA^-deo^- mutants in LB and M9 media. A) Mutants in deoC (Δ), deoA (□) and deoR (○) and wild-type E. coli MG1655 (◇) were treated with 50 μg/ml of TMP in LB medium. Viability was tracked by counting CFU's (on LB plates after 16-hr incubation at 37°C) in the cultures sampled at 1, 2 and 3 hours of the treatment. B) In minimal media with supplements M9TMPAdAA.