The mosquito Aedes aegypti has a large genome size and high transposable element load but contains a low proportion of transposon-specific piRNAs

Table 6 Number of piRNA and siRNA sequences that align to transposon sequences transformed into mosquito germlines.1

piRNA	1	4	2	6	11	12	10
template²	pMos3DB2Her		pBac3EB2Mos		autohermes	pMos3DBhspPBac	Wild
pMos3DB2Her 3,175 (1,839) bp.	1(1)	7(4) ³	0	0	0	0	2(1)
pBac3EB2Mos 3,520 (1,038) bp.	0	0	0	0	0	0	0
autohermes 3,081 (1,830) bp.	0	0	0	0	0	0	0
pMos3DBhspPBac 3,081 (1,785) bp.	0	0	0	0	0	0	0
siRNA
pMos3DB2Her 3,175 (1,839) bp.	0	0	0	0	0	0	0
pBac3EB2Mos 3,520 (1,038) bp.	0	0	1(1)	1(1)	0	0	0
autohermes 3,081 (1,830) bp.	0	0	0	0	0	0	0
pMos3DBhspPBac 3,081 (1,785) bp.	0	0	0	0	0	0	0

¹ All libraries but library #10 were derived from Ae. aegypti lines containing germline transformations of transposon sequences, the name of the transformation plasmid is indicated below each library number. Only matches to the transposon derived portions of the transformation plasmid are reported. Sequences that also match the Ae. aegypti genome assembly are excluded
².Transposon sequence alignment template. Transposon sequences from these plasmids were used as templates for library alignments. Indicated next to each plasmid name is the size (bp) of the transposon derived sequences and in parentheses the size of the transposase sequence.
³ Marked in bold are numbers where the mosquito line is transformed with the alignment template sequence. Numbers in parentheses indicate the number of alignments to the transposase.

ISSN: 1471-2164