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Table 2 Comparison of the frequency of β-signal motif matches in the C-terminal 40 residues compared with the rest of the sequence of MBOMPs

From: Eukaryote-wide sequence analysis of mitochondrial β-barrel outer membrane proteins

Motif pattern

VDAC

Tom40

Sam50

Mdm10

Po x G x x HyxHy

(4/4, 0.20, 1.6 × 10-3)

(5/5, 0.12, 2.5 × 10-5)

(5/5, 0.14, 5.4 × 10-5)

(6/8, 0.17, 4.9 × 10-4)

Po x Ghy x HyxHy

(4/4, 0.09, 6.6 × 10-5)

(5/5, 0.09, 5.9 × 10-6)

(5/5, 0.09, 5.9 × 10-6)

(6/8, 0.07, 2.9 × 10-6)

Po HyGhy x HyxHy

(4/4, 0.07, 2.4 × 10-5)

(4/5, 0.07, 1.1 × 10-4)

(5/5, 0.02, 3.2 × 10-9)

(4/8, 0.04, 1.6 × 10-4)

Po Hy xhy x HyxHy

(4/4, 0.25, 3.9 × 10-3)

(5/5, 0.17, 1.4 × 10-4)

(5/5, 0.12, 2.5 × 10-5)

(5/8, 0.12, 1.0 × 10-3)

P o H y Gh y H y ¯ H y xH y

(4/4, 0.07, 2.4 × 10-5)

(4/5, 0.07, 1.1 × 10-4)

(5/5, 0.00, 0.0)

(4/8, 0.04, 1.6 × 10-4)

P o H y xh y H y ¯ H y xH y

(4/4, 0.21, 1.9 × 10-3)

(5/5, 0.17, 1.4 × 10-4)

(5/5, 0.08, 3.3 × 10-6)

(5/8, 0.12, 1.0 × 10-3)

H y ¯ H y Gh y  x H y xH y

(4/4, 0.11, 1.5 × 10-4)

(4/5, 0.07, 1.1 × 10-4)

(5/5, 0.02, 3.2 × 10-9)

(7/8, 0.04, 1.3 × 10-9)

H y ¯ H y xh y  x H y xH y

(4/4, 0.37, 1.9 × 10-2)

(5/5, 0.23, 6.4 × 10-4)

(5/5, 0.22, 5.2 × 10-4)

(8/8, 0.18, 1.1 × 10-6)

H y ¯ H y Gh y xH y xH y

(4/4, 0.11, 1.5 × 10-4)

(4/5, 0.07, 1.1 × 10-4)

(5/5, 0.00, 0.0)

(7/8, 0.04, 1.3 × 10-9)

H y ¯ H y xh y H y ¯ H y xH y

(4/4, 0.33, 1.2 × 10-2)

(5/5, 0.23, 6.4 × 10-4)

(5/5, 0.20, 3.2 × 10-4)

(8/8, 0.16, 4.3 × 10-7)

  1. Statistics on the frequency of matches in the C-terminal versus non-C-terminal part of MBOMPs families is shown for the 4 known MBOMPs and 10 variations of the β-signal motif. Each cell holds a triple: the fraction of homologs with C-termini which match the motif, the fraction of non-C-terminal length 40 substrings of the MBOMP sequences which match the motif, and a p-value. The p-value is computed with a binomial test, in which the non-C-terminal frequency (e.g. 0.2) is the probability of success and the C-terminal frequency (e.g 4/4) is the observed data.