Identification and functional characterization of small non-coding RNAs in Xanthomonas oryzae pathovar oryzae

Liang, Hong; Zhao, Ying-Tao; Zhang, Jie-Qiong; Wang, Xiu-Jie; Fang, Rong-Xiang; Jia, Yan-Tao

doi:10.1186/1471-2164-12-87

BMC Genomics

Table 4 Differentially expressed proteins in ΔsRNA-Xoo 1 identified by MS

From: Identification and functional characterization of small non-coding RNAs in Xanthomonas oryzae pathovar oryzae

Spot ID	protein name	functions	NCBI acc.no.	mascot score	Sequence coverage	theoretical MW(Da)/pI	Ratio ± SD
Down-regulated protein spots in sRNA- *Xoo* 1 mutant
B1	putative ABC transporter ATP-binding protein	transport	PXO_04409	448	56%	61740/5.37	11.57 ± 4.83
B2	glyceraldehyde-3-phosphate dehydrogenase	metabolism	PXO_02308	529	69%	36134/6.35	4.75 ± 0.57**
B3	argininosuccinate synthase	amino acid biosynthesis	PXO_00352	769	67%	45217/5.66	3.25 ± 0.56
B4	tryptophan repressor binding protein	amino acid metabolism	PXO_03044	438	52%	20167/6.05	2.83 ± 0.18**
B5	3-isopropylmalate isomerase large subunit	amino acid biosynthesis	PXO_02613	314	42%	51984/5.68	1.86 ± 0.21**
B6	N-acetylornithine carbamoyltransferase	amino acid biosynthesis	PXO_00353	314	65%	37582/6.07	1.7 ± 0.19**
B7	superoxide dismutase	oxidation reduction	PXO_00389	343	68%	22703/5.47	1.42 ± 0.03**
B8	septum site-determining protein MinD	replication	PXO_04464	435	46%	28837/5.21	1.57 ± 0.19**
B9	N-ethylammeline chlorohydrolase	metabolism	PXO_00380	263	62%	48943/5.19	ND
B10	2-isopropylmalate synthase	amino acid biosynthesis	PXO_02609	694	71%	53421/5.54	1.54 ± 0.15**
B11	translation elongation factor G	transcription and translation	PXO_04525	642	58%	75924/5.09	4.82 ± 0.42**
B12	dihydrolipoamide dehydrogenase	amino acid metabolism	PXO_01196	582	67%	50445/5.98	2.29 ± 0.54
B13	N-acylglucosamine 2-epimerase	metabolism	PXO_01231	811	77%	46884/5.27	2.23 ± 0.34**
B14	ATP synthase subunit alpha	transport	PXO_03111	777	48%	55391/5.38	2.23 ± 0.5
B15	phosphoenolpyruvate synthase	Pyruvate metabolism	PXO_00922	622	48%	86689/5.16	6.36 ± 1.69
B16	Polyribonucleotide nucleotidyltransferase	metabolism	PXO_01307	729	55%	75502/5.47	5.39 ± 0.93
Up-regulated protein spots in ΔsRNA- *Xoo* 1 mutant
B17	pyrroline-5-carboxylate reductase	amino acid metabolism	PXO_01991	301	51%	29364/4.93	1.48 ± 0.07**
B18	peptide deformylase	protein synthesis	PXO_04055	379	55%	17129/5.29	1.45 ± 0.02**
B19	outer membrane protein	transport	PXO_03097	343	32%	23707/5.27	1.43 ± 0.04**
B20	Chaperone protein dnaJ	DNA replication	PXO_01186	702	64%	41079/6.21	1.52 ± 0.14**
B21	hydrolase, carbon-nitrogen family	metabolism	PXO_06060	526	68%	30229/5.89	1.57 ± 0.12**
B22	hypothetical protein	hypothetical protein	PXO_01766	635	75%	16502/6.3	1.41 ± 0.01

ND: not detectable in the 2-DE maps of wild-type or ΔsRNA-Xoo 1 mutant. SD: Standard Deviation. Statistical significance was determined using Student's two-tailed t test for unpaired means P < 0.05, P < 0.01**.

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ISSN: 1471-2164

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