Figure 4

Inactivation of putative xylR CAC2613 in ATCC 824 leaded to faster xylose utilization in C. acetobutylicum. A: location of CAC2613 in C. acetobutylicum and xylR in different organisms; B: Alignment of CAC2613 and xylR in different organisms; C: CAC2613 inactivation in ATCC 824 using Targetron system; D: Accurate pH-controlled fermentation profile of mutant strain versus wild type in 6% xylose contained P2 medium.