Line | Size-range | Number of fragments | Genome fraction | Sequenced (32 bp reads) | RRL coverage calculated |
---|
Layers
| 150-200 | 583826 | 101 Mb (8%) | 18.7 Mb (1.5%) | 37-40X |
Broilers
| 125-200 | 947538 | 151 Mb (12%) | 30.3 Mb (2.4%) | 22-26X |
- Fragments were collected in corresponding size ranges as used in the in vitro RRL preparation. The total number of collected fragments and number of bases captured are indicators of what genome fraction was sampled. Based on trimmed reads, the fraction of the genome actually sequenced was calculated. The number of raw read pairs obtained (see Table 1) divided by the number of fragments is an indicator of the RRL coverage.