Table 3 Transcriptome coverage estimates: ESTcalc
From: De novo characterization of the gametophyte transcriptome in bracken fern, Pteridium aquilinum
Input Parameters | ESTcalc estimate | Actual |
|---|---|---|
Number of technologies | 1 | 1 |
Technology | 454 GS-FLX | 454 GS-FLX (Titanium) |
Library type | normalized | normalized |
MB/Plate | 254 | 254.0076 |
Reads/Plate | 681,722 | 681,722 |
BP/Read (mean) | 372.6 | 372.6 |
Predicted assembly statistics | Â | Â |
Total Assembled Sequence (MB) | 26.2 | 30.97 |
Unigene count | 32,044 | 56,256 |
Mean unigene length (bp) | 819 | 547.23 |
Mean unigene length (longest unigene per gene, bp) | 1,143 | -- |
Singleton yield (%) | 19 | 17.2400 |
Percent transcriptome (%) | 87 | -- |
Percent of genes tagged (%) | 100 | -- |
Percent of genes with 90% coverage (%) | 69.8 | -- |
Percent of genes with 90% coverage by largest unigene (%) | 56.4 | -- |
Percent of genes with 100% coverage (%) | 23.7 | -- |
Percent of genes with 100% coverage by largest unigene (%) | 22.2 | -- |