A nuclear magnetic resonance based approach to accurate functional annotation of putative enzymes in the methanogen Methanosarcina acetivorans

Table 1 Summary of MA genes studied

MA Gene	IMG Annotation¹	Experimental data consistent with IMG annotation?	Comment²
MA0154 (350 aa)	Biotin synthase	No	Revised annotation is to pyrrolysine biosynthesis protein
MA0246 (422 aa)	4-Hydroxybenzoate decarboxylase	No	Revised annotation is 3-octaprenyl-4-hydroxybenzoate carboxylyase
MA0940 (183 aa)	Conserved hypothetical	-	Revised annotation is alpha-ribazole-5’-phosphate phosphatase and experimental data is consistent with this
MA2498 (196 aa)	Fumarate hydratase	Yes	Revised annotation is fumarate hydratase/tartrate dehydratase but experimental data is only consistent with the former
MA3520 (412 aa)	Glycine hydroxymethyl-transferase	Yes	No change in annotation
MA3706 (184 aa)	Ham1 protein/nucleotide triphosphatase	Yes	No change in annotation
MA4265 (342 aa)	Isocitrate/ isopropylmalate dehydrogenase family protein	Partially	Revised annotation is isocitrate/ isopropylmalate dehydrogenase but the experimental data is only consistent with the former

¹ Obtained from the DOE Integrated Microbial Genomes database (http://img.jgi.doe.gov/); ² Revised annotations were based on comparison with the updated annotations in M. burtonii[13] as described in the text.

ISSN: 1471-2164