AlgaGEM – a genome-scale metabolic reconstruction of algae based on the Chlamydomonas reinhardtii genome

BMC Genomics

Table 3 Minimal set of constraints imposed to represent different growth condition

Inputs, outputs and constraints	Autotrophic	Heterotrophic	Mixotrophic
C source: CO₂ uptake	+	-	+
C source: Acetate uptake	-	+	+
Photons uptake (free flux)	+	-	+
Optimization 1: minimize uptake of	Photons	Acetate	Photons
Optimization 2: maximize product	H₂	H₂	H₂
Biomass rate (fixed) *	0.059 h^-1	0.035 h^-1	0.066 h^-1

*Biomass rate and biomass equation was used for each growth regime, based on measurements found in the literature [13].

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