Quantitative genome re-sequencing defines multiple mutations conferring chloroquine resistance in rodent malaria

BMC Genomics

Table 2 Illumina^® whole-genome re-sequencing - Confirmed and High-Confidence Mutations in AS-30CQ

Chr		Nucleotide number	WTSI	AS-30CQ	Quality Score	Mutation	Gene (abbrev)	Nearest Gene	P. falciparum orthologue
	POINT MUTATIONS
2		216,954	C	A	99	V2728F	PCHAS_020720		PFA0220w
3		70,553	G	T	99	T707N	PCHAS_030200		none
3		474,123	C	A	99	T719	PCHAS_031370		PFB0675w
7		994,546	G	A	99	S106N	PCHAS_072830		PFD0830w
10		634,932	T	C	99	Y162H	PCHAS_101550		PF14_0279
11		996,332	G	T	99	A173E	PCHAS_112780		PFF1430c
14		936,945	T	G	92			PCHAS_142600	PF08_0081
	DELETIONS
	Start (nucleotide number)	Finish (nucleotide number)
5	683,724	684,989					PCHAS_051920		none
7	876,909	876,914						PCHAS_072420	PF08_0067

Summary of the high confidence mutations proposed in clone AS-30CQ (see Additional File 1). Read depth and quality scores fro mutations were according to SSAHA2. Start and Finish nucleotides for deletions are approximate only.

ISSN: 1471-2164