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Figure 6 | BMC Genomics

Figure 6

From: Identification of microRNAs from Amur grape (vitis amurensis Rupr.) by deep sequencing and analysis of microRNA variations with bioinformatics

Figure 6

Mapping of mRNA cleavage sites by RNA ligase-mediated 5' RACE. Each top strand (black) depicts a miRNA-complementary site in the target mRNA, and each bottom strand (pink) depicts the miRNA. Watson-Crick pairing (vertical dashes), G:U wobble pairing (circles) and mismatched bases pairing (X) are indicated. Arrows indicate the 5' termini of mRNA fragments isolated from Amur grape, as identified by cloned 5'RACE products, with the frequency of clones shown. Only cloned sequences that matched the correct gene and had 5' ends within a 100 nt window centered on the miRNA complementary site are counted. Partial mRNA sequences from target genes were aligned with the miRNAs. Numbers indicate the fraction of cloned PCR products terminating at different positions.

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BMC Genomics

ISSN: 1471-2164

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