Table 4 List of identified non-conserved miRNAs in Amur grape

va-miR001^a

TTTTTTTATTGGATCCGTCGGGA

108

84

13:9154663

-21.4

AM473732.1;

AM473732.1;

GU363535.1

va-miR002

TTTTGTTTTTATTGTTGTTTT

14

240

16:6817573

-42.05

AM432096.2;

AM436712.2

va-miR003

TTTTCTTTTCCTGCATTTTCT

21

196

10_random:812676

-28.77

AM454974.2

va-miR004

TTTTCCTATGATTTCTTGGCA

18

139

17:4715183

-44.23

AM451502.1

va-miR005

TTTGCATGAGGGGGGATTGTCAT

8

160

14:10210052

-55.9

AM425582.2

va-miR006^a -1

TTTCTTAGCAACCAAACAGAG

10

213

3:3982162

-36.6

AM453475.2

AC210553.1

va-miR006^a -2

TTTCTTAGCAACCAAACAGAG

8

213

3:3995013

-36.6

AM453475.2

AC210553.1

va-miR006^a -3

TTTCTTAGCAACCAAACAGAG

8

255

19:4998280

-74.2

AM453475.2

AC210553.1

va-miR007 ^b

TTTCCGACTCGCACTCATGCCGT

812

103

17:5737883

-49

No

Vv-miR076^c

Y

va-miR008 ^a

TTTCCACGGCTTTCTTGAACT

16,273

182

1:1942578

-57.1

AM481354.2

BT117687.1

Vv-miR075^c

Y

va-miR009

TTTCCACATCTTTCTTGAACT

10

113

12:7648535

-49.5

AM480580.2

Y

va-miR010 ^a

TTTAATTTAAATATTGAAGAT

13

220

8:10844562

-43.6

no

AC216916.1;

AC212928.1

va-miR011^b

TTGTCGCAGGAGAGACGGCACT

6

92

14:17492727

-57.8

AM479856.1

MirC6^d

Y

va-miR012

TTGGCACATTATCTAACAACT

14

107

8:2707092

-21.1

No

va-miR013

TTGCTGAGAGAGTCGTCTGCC

53

81

9:13023036

-38.5

No

Y

va-miR014

TTGCTAAATCTTTGATTCGATC

20

103

13:10216545

-37.52

AM443747.2

va-miR015

TTGCTAAATCATTGATTCGATC

11

101

13:10213352

-34.2

AM439308.2;

AM447569.2

Y

va-miR016

TTCTTGTGATCTTGTTGTTTC

9,441

148

5:20754701

-88.9

AM454776.2

va-miR017

TTCTTCTTAACGTCTGACTTA

10

135

1:1002143

-57.6

AM448689.1

va-miR018 ^a

TTCTCGGACCAGGCTTCATTC

148

161

7:14570131

-64.3

no

BT089825.1

Y

va-miR019

TTCAAGTCAAAGTCGAACAAG

16

71

1:15194533

-20.2

no

va-miR020

TTATTAACCATTTAAATTTA

5

212

4:14934239

-21.55

AM458370.2

va-miR021^b

TTATGTGAGTGTTCGGCAAATC

16

90

5:20305533

-37.9

no

Vv-miR073^c

va-miR022-1

TTAGTTTGAACTAGGAGATGACA

10

113

10:6246751

-20.8

no

va-miR022-2

TTAGTTTGAACTAGGAGATGACA

10

255

10:6328614

-49.9

no

va-miR023 ^b

TTAGATGATCATCAACAAACA

9,128

118

5:23154645

-45.5

AM429664.2

Vv-miR072^c

Y

va-miR024

TTACTTTTATCTGAATAGAAA

8

108

15:3408926

-26.7

AM465770.2

va-miR025 ^b

TTACACAGAGAGATGACGGTGG

633

105

5:6660435

-50.04

AM429841.2

MirC22^d

Y

va-miR026

TGGTAAATTGGTTTAAATATTC

11

192

18:19596668

-22.4

AM487707.2;

AM486523.2;

AM442872.2;

AM465537.1

va-miR027 ^a

TGGATGCATGTAGCTTGTCAA

21

103

18:4024355

-71.9

AM484186.2;

AK329204.1;

XM_002892267.1

Y

va-miR028

TGGATACACTTTTTATTTTTT

6

204

10:2463091

-29.5

AM467500.2;

AM425302.2;

AM476399.1

va-miR029^b -1

TGGAGAAGGGGAGCACGTGCA

5

118

14:1404606

-59

AM462494.2;

Vv-miR068^c

va-miR029^b -2

TGGAGAAGGGGAGCACGTGCA

6

119

7:3651397

-45.9

AM462494.2;

Vv-miR068^c

va-miR030

CGCCGCTCTCCTGTGACAAGA

6

92

14:17492727

-57.8

AM479856.1

va-miR031^b

TGCATTTGCACCTGCACCTTA

104

165

6:22063225

-75.22

No

Vv-miR020*^c

Y

va-miR032

TGATTGTAGAAAATGTTTTTAAC

5

111

6:21035897

-36.7

AM439539.1

va-miR033

TGATATTAGCAGCTGAGAACA

10

147

14:11536480

-70

AM449587.2

Y

va-miR034 ^b

TGAGTAGTGGACTATCGCATG

9

116

17:7362816

-43.9

AM489347.2;

AM461823.2;

Vv-miR065^c

va-miR035

TGAGGAAGGGTGTTAGAGTAC

13

90

13:5595054

-46.1

No

va-miR036 ^b

TGACCGGCTCTTATCTCTCATG

27

189

17:351716

-79.3

AM447150.1

Vv-miR063^c

Y

va-miR037

TGACACTATATAAATATGAA

6

244

2:8067706

-48.1

AM430446.2;

AM466868.2;

va-miR038

TGAATGGTTGGTAAATTGAG

11

150

12:14954776

-40.6

AM482866.2;

AM466422.2;

va-miR039 ^b

TGAAATGTAGGCAAGGAAAAG

9

192

16:8071148

-53.6

AM478700.2;

Vv-miR059^c

va-miR040

TCTGTTTGGACGCCGAGAAAA

7

217

17:10557309

-62.22

No

va-miR041^b

TCTGTCGCAGGAGAGATGATGC

30

103

14:17809215

-53.9

No

MirC23^d

Y

va-miR042

TCTGTACTTTAAGAATCTGGCTT

15

208

15:6195235

-41.2

AM454626.2

Y

va-miR043

TCTGCATTTGCACCTGCACCT

15

165

8:17999572

-57.1

No

va-miR044

TCTCTTGATATTAGTAGCTGA

9

138

14:11536485

-71.9

No

va-miR045

TCGAGTGGGAACGCATTGAGC

6

150

12:13192937

-35.7

AM486659.2

va-miR046^b

TCCCAGGAGAGATGGCACCTGC

330

85

17:5984990

-43

AM457000.2

Vv-miR055^c

va-miR047 ^a

TCACAAGTTCATCCAAGCACCA

32,758

136

18_random:5237347

-51.87

No

EZ339498.1

Vv-miR054^c

Y

va-miR048

TCAATTAGTAGCTTAACATGG

8

132

11:5115765

-58.49

AM471165.2

va-miR049^b

TCAATAAGGTACTTTTAGCT

108

126

11:12939237

-34.3

AM487033.2

Vv-miR053^c

Y

va-miR050

TCAACTTGATTTTTGGATGAG

9

101

8:15743876

-27.8

AM460148.2

va-miR051

TCAAAAGAGAAAATGTGGATG

46

100

6:4462385

-35.9

AM428913.2

va-miR052

TATTAAGTTCAAGTGAAAATT

5

206

10_random:1196749

-64.5

AM473600.2

va-miR053

TAGTGATTTTAGGAAGCGTTT

7

271

16:432019

-45.26

AM455590.2

va-miR054 ^b

TAGGAAACGTTTCTACTCTTT

20

311

scaffold_1047:19353

-59.7

No

Vv-miR051^c

va-miR055 ^b

TAATCTGCATCCTGAGGTCTA

48

107

8:14329491

-44.9

AM426746.2

Vv-miR048^c

Y

va-miR056-1

TAAAATCACTATCAAACGAA

15

133

10:1636145

-21

AM454228.2

va-miR056-2

TAAAATCACTATCAAACGAA

13

134

11:1222860

-21.2

AM454228.2

Y

va-miR056-3

TAAAATCACTATCAAACGAA

12

132

14:9946665

-25.85

AM454228.2

va-miR056-4

TAAAATCACTATCAAACGAA

13

255

16_random:3940513

-39.5

AM454228.2

va-miR056-5

TAAAATCACTATCAAACGAA

15

136

8:11389633

-33.7

AM454228.2

va-miR057^b -1

GTTGGAAGTCGGTGGGGGACC

7,884

91

19:13455035

-47.9

No

Vv-miR047^c

Y

va-miR057^b -2

GTTGGAAGTCGGTGGGGGACC

7,884

92

19_random:737237

-42.6

No

Vv-miR047^c

Y

va-miR058

GTAGCATCATCAAGATTCACA

14

108

6:21821956

-46.5

AM439514.2

va-miR059 ^a

GGAATCTTGATGATGCTGCAT

18

152

6:5961963

-69.27

No

GQ905588.1;

GQ419177.2

Vv-miR038*^c

Y

va-miR060

GAACATCCAGATCATAGGTATGA

9

276

13:6835040

-49.2

AM470549.2

va-miR061

CTTCAGTGAGTGTGGCGTCAACG

8

155

1_random:4350301

-56.26

AM424902.2

va-miR062

CTATGTTATAGGATCTTGGAT

315

99

10:1456140

-51.8

No

Y

va-miR063^b

CTAGGAAGCGTTTTTAATATT

6

99

8:910651

-38.93

AM439566.1

Vv-miR035^c

va-miR064

CTAAAATCATTGTCAAACGAG

12

303

scaffold_352:50742

-54.8

AM449536.2

va-miR065-1

CTAAAATCACTACCAAACGG

10

99

1:15345941

-23

AM432849.2

va-miR065-2

CTAAAATCACTACCAAACGG

9

129

7:12853717

-24.2

AM432849.2

va-miR066

CGTCTTTTAAATTTTTTTATC

6

121

7:11538839

-19.7

No

va-miR067

CGGGAGATGACTACTGGAAG

8

106

17:2382956

-60.1

No

va-miR068 ^a

CGCTATCCATCCTGAGTTTCA

375

122

8:8483671

-54.4

No

AC235430.1;

FM163968.1;

FM163967.1

Y

va-miR069

CGCCGCTCTCCTGTGACAAGA

6

92

14:17492727

-57.8

AM479856.1

va-miR070

CCAAGAGGGTGGAGTTCAGA

10

111

14:13763190

-54.9

No

va-miR071

CATGTGCCCCTCTTCCCCATC

5

168

8:8981156

-60.21

AM459087.2

va-miR072

CATGGGCGGTTTGGTAAGAGG

26,555

117

1:3849422

-46.2

No

Y

va-miR073

CATGACAAAAGATACTTCATT

19

95

6:9332049

-64.1

No

va-miR074

CAGGTTTATTGTTTTTTAATT

7

95

1_random:3034469+

-22

No

va-miR075

CAGGAAAGTCAGGGAAGTGTA

9

93

13:6656017

-41.93

No

Y

va-miR076

CAAGTGTGGGATTTTGGGTGGCT

14

187

4:15148566

-59.2

AM475853.2

va-miR077

ATTTCATCATTATATAAAACG

5

260

2:10655532

-124.6

No

va-miR078

ATTGACATCGTCTAACATAA

27

96

13:908030

-37.2

No

va-miR079

ATTATTTTTTTTCATGGGAA

7

183

6:4945482

-40.4

AM435008.2

va-miR080

ATTATATGTGTGAGCCGTTCA

39

97

19:13648253

-23.2

No

va-miR081

ATCATGTAGATATTGTCCGCT

21

216

18:11023560

-77.4

No

va-miR082 ^b

ATATTATTAGGGAATTAAGT

4

82

18:15863697

-19.7

AM482601.2

Vv-miR024^c

va-miR083

ATACCATGTGGAAAAGAGGAATC

89

199

16:2126243

-38.7

AM430285.2

va-miR084

ATAAAACAGGGCATAATTAG

90

162

8:3276798

-27.3

AM455775.2

Vv-miR022^c

va-miR085-1

AGGGAAGGTCCCGTTGAGTT

8

87

3:4651805

-34.8

AM432941.2

va-miR085-2

AGGGAAGGTCCCGTTGAGTT

11

80

scaffold_389:33261

-31

AM432941.2

va-miR086

AGAGCCAAATATACTCCGCGG

19

83

9:402800

-52.1

AM471736.2

va-miR087^b -1

AGAATGTGTTTGATAGTAATT

7

220

13:10609418

-32.71

AM450684.2;

AM470125.2;

AM466462.2

Vv-miR013^c

va-miR087^b -2

AGAATGTGTTTGATAGTAATT

7

152

3:9026582

-32.9

AM450684.2;

AM470125.2;

AM466462.2

Vv-miR013^c

va-miR087^b -3

AGAATGTGTTTGATAGTAATT

7

134

5:8615567

-39.8

AM450684.2;

AM470125.2;

AM466462.2

Vv-miR013^c

va-miR087^b -4

AGAATGTGTTTGATAGTAATT

7

99

7:6125455

-19.4

AM450684.2;

AM470125.2;

AM466462.2

Vv-miR013^c

va-miR088^a

AGAAGAGAGAGAGTACAGCTA

27

259

5:17354630

-65.6

No

AL606589.3;

AY946858.1

Vv-miR012^c

va-miR089

AGAAGAACAAGTAGACTGAGC

11

95

12:17274522

-35.6

AM457953.2

va-miR090

AGAAAACTGTTTTTAAAAACT

25

209

3:3268552

-33.5

AM428594.2

va-miR091

AAGTGGTGCTGTCTAGGGTAT

8

153

16_random:2298331

-74.9

AM461957.1

va-miR092

AAGGTTACGAAGAATGTGAG

7

65

6:20373849

-28.4

AM449474.1

Y

va-miR093 ^b

AAGGGTTTCTCACAGAGTTTA

40

135

18:1385784

-66.9

AM428484.2

Vv-miR079^c

va-miR094 ^b

AACTAACATAACTAAAGTGAA

21

78

18:7824675

-25

AM444284.2

Vv-miR004^c

va-miR095

AACAAATGCTTGATTAAATG

7

185

16:3892094

-46.34

AM456738.2

va-miR096

AAATTTGATTTTATGGTATT

14

277

1:4778703

-52.3

AM479127.2

va-miR097

AAATTGGCTCTGTAAATTTC

14

137

18:3503665

-77.6

AM454446.1

Y

va-miR098

AAATTGACCTATTTAATAACT

6

225

13:6487398

-33.8

No

va-miR099-1

AAATAGAACAAAACACAGCAA

8

101

4:15872785

-57.2

AM424238.2

va-miR099-2

AAATAGAACAAAACACAGCAA

8

101

scaffold_477:40535

-63.2

AM424238.2

va-miR100

AAAGTGCATTTGATAGTGATTC

5

142

19_random:164412

-29.22

AM481492.2

Y

va-miR101

AAAAATGAAAATGGGAGTCGGT

11

243

5:12357599

-42.41

AM466362.2

va-miR102 ^b

AAAAAGTTTGTCAAATATTG

15

97

19:13648253

-23.2

AM435471.2

Vv-miR001^c

va-miR103

AAAAAATTCAAAGGGAAATC

8

162

2:2995078

-26.63

AM473477.2

va-miR104 ^b

GGAATGGATGGTTAGGAGAG

2,136

17:5578270

-54.4

AM458290.1

MirC13^d

Y

va-miR105

TAAAAGATAGGGATCGGATGGAG

6

192

14:15112983

-59.2

AM463372.2

va-miR106

GTTGGAAGTCGGTGGGGGACC

4,330

91

19:13455035

-47.9

AM481325.2