Figure 3

Temporal expression patterns of 4,484 Brugia transcripts that are differentially expressed (FDR 5%) observed over the entire life cycle. Five clusters were identified by K-means clustering based on their temporal expression patterns. The average expression pattern of the transcripts in each cluster is indicated by a dark blue line. For visualization purposes, each transcript estimated mean log-scale expression profile was standardized to have a mean of 0 and a variance of 2 prior to plotting. A, Cluster one; B, Cluster two; C, Cluster three; D, Cluster four; E, Cluster five. Mf, microfilariae; AF, adult females; AM, adult males; 6WF, 6 week females; 6WM, 6 week males; L4, L4 stage, 2 week post infection; L3, infective third stage larvae.