Table 2 Protein identification of 30 spots with highest Mascot scores picked on deep purple stained 2D-gel
Spot Nb | Fold changes | Vectorbase access number | Mascot score | Top ranking match | Predicted PI | Predicted mass (kDa) | % sequence coverage | Species | |
|---|---|---|---|---|---|---|---|---|---|
Proteases | |||||||||
2 | − | 1.88 | AAEL015386_a | 249 | dipeptidyl-peptidase | 4.91 | 84.9 | 42 | Ae. aegypti |
3 | − | 2.77 | AAEL015386_b | 149 | dipeptidyl-peptidase | 4.91 | 84.9 | 22 | Ae. aegypti |
4 | + | 1.81 | AAEL012774_a | 237 | protease m1 zinc metalloprotease | 4.81 | 102.5 | 41 | Ae. aegypti |
5 | + | 2.34 | AAEL012774_b | 162 | protease m1 zinc metalloprotease | 4.81 | 86.7 | 42 | Ae. aegypti |
20 | + | 1.81 | AAEL012776_a | 135 | protease m1 zinc metalloprotease | 5.19 | 103.3 | 30 | Ae. aegypti |
21 | − | 2.74 | AAEL012776_b | 146 | protease m1 zinc metalloprotease | 5.19 | 103.3 | 27 | Ae. aegypti |
19 | − | 3.81 | AAEL012776_c | 136 | protease m1 zinc metalloprotease | 5.19 | 103.3 | 22 | Ae. aegypti |
Detoxification enzymes | |||||||||
45 | + | 3.01 | CPIJ019700 | 119 | cytochrome P450 | 7.6 | 58.4 | 41 | C. quinquefasciatus |
Kinases-Phosphatases | |||||||||
22 | − | 1.58 | AAEL003313 | 62 | alkaline phosphatase | 5.46 | 61.0 | 23 | Ae. aegypti |
24 | − | 1.92 | AAEL003298_a | 186 | alkaline phosphatase | 5.28 | 58.8 | 39 | Ae. aegypti |
25 | − | 1.16 | AAEL003298_b | 194 | alkaline phosphatase | 5.23 | 58.3 | 39 | Ae. aegypti |
Other enzymes | |||||||||
6 | + | 1.78 | AAEL010532 | 146 | alpha-amylase | 4.82 | 68.9 | 37 | Ae. aegypti |
13 | − | 1.27 | AAEL004580 | 129 | beta-galactosidase | 4.87 | 74.1 | 34 | Ae. aegypti |
11 | − | 2.06 | AAEL002827_a | 61 | ATP synthase beta subunit | 5.03 | 53.9 | 32 | Ae. aegypti |
23 | − | 2.29 | AAEL002827_b | 152 | ATP synthase beta subunit | 5.03 | 53.9 | 55 | Ae. aegypti |
16 | − | 2.06 | AAEL008787_a | 162 | V-ATP synthase subunit alpha | 5.26 | 68.5 | 31 | Ae. aegypti |
17 | − | 2.19 | AAEL008787_b | 231 | V-ATP synthase subunit alpha | 5.26 | 68.5 | 42 | Ae. aegypti |
18 | − | 1.82 | AAEL008787_c | 132 | V-ATP synthase subunit alpha | 5.26 | 68.5 | 30 | Ae. aegypti |
27 | − | 1.51 | AAEL005798_a | 200 | V-ATP synthase subunit beta | 5.31 | 54.8 | 49 | Ae. aegypti |
28 | − | 1.69 | AAEL005798_b | 177 | V-ATP synthase subunit beta | 5.31 | 54.8 | 44 | Ae. aegypti |
29 | − | 1.72 | AAEL005798_c | 187 | V-ATP synthase subunit beta | 5.31 | 55.4 | 53 | Ae. aegypti |
30 | − | 2.06 | AAEL005798_d | 229 | V-ATP synthase subunit beta | 5.38 | 55.5 | 52 | Ae. aegypti |
31 | − | 2.39 | AAEL005798_e | 197 | V-ATP synthase subunit beta | 5.38 | 55.4 | 48 | Ae. aegypti |
32 | − | 1.89 | AAEL005798_f | 181 | V-ATP synthase subunit beta | 5.38 | 55.4 | 56 | Ae. aegypti |
52 | − | 2.14 | AAEL012035_a | 93 | V-ATP synthase subunit E | 5.91 | 25.7 | 35 | Ae. aegypti |
53 | − | 2.21 | AAEL012035_b | 88 | V-ATP synthase subunit E | 5.91 | 25.7 | 38 | Ae. aegypti |
Other functions | |||||||||
8 | + | 10.74 | AAEL001005_a | 195 | calreticulin | 4.42 | 47.0 | 43 | Ae. aegypti |
8 | − | 2.56 | AAEL001005_a | 226 | calreticulin | 4.42 | 47.0 | 49 | Ae. aegypti |
9 | + | 3.14 | AAEL001005_b | 210 | calreticulin | 4.42 | 47.0 | 49 | Ae. aegypti |
10 | + | 2.75 | AAEL001005_c | 158 | calreticulin | 4.42 | 47.0 | 48 | Ae. aegypti |
12 | + | 3.01 | AAEL001005_d | 93 | calreticulin | 4.42 | 46.7 | 35 | Ae. aegypti |