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Table 3 Insertions in genes important for growth or virulence

From: IS-seq: a novel high throughput survey of in vivo IS6110 transposition in multiple Mycobacterium tuberculosis genomes

Locus

Gene ID

Description

IS position within ORF (%)1

PCR verification2

Reference

Essential genes

Rv0336

-

Conserved 13E12 repeat family protein

71.2

+

[25]

Rv0405

pks6

Probable membrane bound polyketide synthase

26.7

+

[26]

Rv1371

-

Probable conserved membrane protein

17.8

+

[29]

Rv1469

ctpD

Probable cation transporter P-type ATPase D

2.5

+

[29]

Rv1477

ripA

Peptidoglycan hydrolase

72.9

+

[28, 30]

Rv1753c

PPE24

PPE Family protein

2.4

+

[26],[25][27]

Rv1978

-

Conserved hypothetical protein

41.8

+

[27]

Rv2388c

hemN

Coproporphyrinogen III oxidase

28.7

-

[29],[27]

Rv2708c

-

Conserved hypothetical protein

61.8

+

[27]

Rv2808

-

Hypothetical protein

23.6

+

[29]

Rv2812

-

Probable transposase

8.8

+

[28, 30]

Rv2817c

-

Conserved hypothetical protein

71.8

+

[28, 30]

Rv3018c

PPE46

PPE family protein

4.7

+

[28, 30]

Rv3112

moaD1

Probable molybdenum cofactor biosynthesis protein D

29.8

+

[28, 30]

Rv3113

-

Possible phosphatase

34.1

+

[28, 30]

Rv3114

-

Conserved hypothetical protein

40.7

-

[29]

Rv3201c

-

Probable ATP-dependent DNA helicase

0.2

+

[28, 30]

Rv3229c

desA3

Possible linoleoyl-CoA desaturase

43.4

+

[29],[27]

Rv3343c

PPE54

PPE family protein

9.3

+

[28, 30]

Rv3376

-

Conserved hypothetical protein

60.9

+

[27]

Essential genes - Less probable to affect function

Rv0001

dnaA

Chromosomal replication initiation protein

95.1

+

[28, 30]

Rv0755c

PPE12

PPE Family protein

96.4

+

[28, 30]

Rv2282c

-

Probable transcription regulator (LysR family)

84.9

+

[27]

Rv2833c

ugpB

Probable Sn-glycerol-3-phosphate-binding lipoprotein

88.9

+

[28, 30]

Rv2856

nicT

Possible nickel-transport integral membrane protein

89.8

+

[28, 30]

Rv3111

moaC1

Molybdenum cofactor biosynthesis protein C

92.8

-

[28, 30]

Rv3177

-

Possible peroxidase

90.4

+

[28, 30]

Virulence, detoxification and adaptation category (Tuberculist)

Rv0591

mce2C

MCE-family protein

56.1

  

Rv1477

ripA

Peptidoglycan hydrolase

72.9

  

Rv1720c

vapC12

Possible toxin

7.9

  

Rv2494

-

Conserved hypothetical protein

97.2

  

Rv3176c

mesT

Probable epoxide hydrolase

11.8

  

Rv3177

-

Possible peroxidase

90.4

  

Rv3473c (MT3579

bpoA

Possible peroxidase

93.8

  
  1. 1) Indicates the position of the insertion within the ORF as the percentage of gene upstream of the insertion.
  2. 2) Insertions verified using PCR: +, PCR positive; -, PCR negative.
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BMC Genomics

ISSN: 1471-2164

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