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Table 1 Effect of stress on haematological variables 1 in control and weaned calves

From: Transcriptomic analysis of the stress response to weaning at housing in bovine leukocytes using RNA-seq technology

  

Days post weaning

P-values

Variable

 

0

1

2

3

7

T

S

T × S

Total Leukocytes

C

13.3 ± 0.97

13.8 ± 1.44

13.9 ± 0.71

15.6bx ± 1.08

13.9x ± 0.61

NS

NS

*

(×103 cells/μL)

W

12.2 ± 0.91

13.2 ± 1.34

12.1 ± 0.66

12.2y ± 1.01

11.3y ± 0.57

   

Neutrophils, %

C

23.7 ± 2.63

24.9x ± 2.30

28.9 ± 2.92

34.9b ± 3.12

28.9 ± 2.29

NS

NS

***

 

W

30.9 ± 2.45

43.4cy ± 2.14

29.7 ± 2.72

30.1 ± 2.90

26.3 ± 2.14

   

Lymphocytes, %

C

63.7 ± 2.87

63.9x ± 2.17

58.3 ± 2.44

53.3b ± 2.59

59.0 ± 2.50

NS

NS

***

 

W

58.4 ± 2.67

44.9cy ± 2.02

58.8 ± 2.27

57.8 ± 2.41

64.1 ± 2.33

   

N:L Ratio

C

0.38 ± 0.076

0.41x ± 0.061

0.52 ± 0.079

0.69b ± 0.098

0.51 ± 0.056

NS

NS

***

 

W

0.57 ± 0.071

0.99cy ± 0.057

0.52 ± 0.074

0.54 ± 0.091

0.42 ± 0.052

   

Eosinophils, %

C

2.7 ± 0.46

1.9a ± 0.23

3.4 ± 0.86

3.7 ± 0.89

3.5 ± 1.18

NS

NS

NS

 

W

1.6 ± 0.43

1.9 ± 0.21

2.3 ± 0.79

1.9 ± 0.83

1.8 ± 1.09

   

Monocytes, %

C

6.6 ± 0.36

7.1 ± 0.44

7.2 ± 0.39

6.1 ± 0.63

5.9 ± 0.45

NS

NS

*

 

W

7.5 ± 0.33

6.9 ± 0.41

6.9 ± 0.36

7.5 ± 0.59

5.7b ± 0.42

   

RBC

C

11.9 ± 0.21

11.8 ± 0.19

12.3b ± 0.24

12.1 ± 0.27

12.1 ± 0.28

NS

NS

NS

(×106 cells/μL)

W

11.6 ± 0.22

11.6 ± 0.21

11.8 ± 0.26

11.8 ± 0.29

11.8 ± 0.29

   

HCT, %

C

34.6 ± 0.68

34.4 ± 0.69

35.9b ± 0.84

35.5 ± 0.85

35.3 ± 0.81

NS

*

NS

 

W

33.5 ± 0.64

33.4 ± 0.65

34.1 ± 0.78

34.5 ± 0.79

34.2 ± 0.76

   

HGB (g/dL)

C

13.0 ± 0.19

13.1 ± 0.25

13.5b ± 0.28

13.4 ± 0.29

13.4 ± 0.29

NS

*

NS

 

W

12.7 ± 0.19

12.8 ± 0.23

12.9 ± 0.26

12.9 ± 0.28

12.9 ± 0.27

   

MCHC (g/dL)

C

37.7 ± 0.28

38.1b ± 0.24

37.7 ± 0.22

37.7 ± 0.23

37.9 ± 0.29

NS

NS

*

 

W

37.9 ± 0.26

38.5c ± 0.22

37.8 ±0.21

37.5a ± 0.21

37.7 ± 0.27

   

Platelet number

C

640.1 ± 63.06

697.2x ± 60.73

700.4 ± 55.59

684.8 ± 57.03

665.2 ± 49.91

NS

*

NS

(×103 cells/μL)

W

604.5 ± 58.79

469.4ay ± 56.62

596.4 ± 51.83

562.3 ± 53.17

536.3 ± 46.54

   
  1. 1Haematological variables; total leukocyte, neutrophil and lymphocyte percentage, neutrophil:lymphocyte (N:L) ratio, eosinophil and monocyte percentage, red blood cell (RBC) number, haematocrit (HCT) percentage, haemoglobin (HGB) and mean corpuscular haemoglobin concentration (MCHC). The values are expressed as least squares means (Lsmeans) ± s.e.
  2. C = Control, W = Weaned, T = treatment, S = sampling time, T × S = treatment × sampling time interaction, NS = not significant (P > 0.05).
  3. * = P < 0.05, ** = P < 0.01, *** = P < 0.001.
  4. a,b,cWithin rows, Lsmeans differ from pre-weaning baseline by P < 0.05, P < 0.01 and P < 0.001, respectively.
  5. x,yBetween rows, Lsmeans differ between treatments by P < 0.05.