Figure 2

Basal gene expression polymorphism. Relative mRNA abundances of four hemocyte genes in eight individual oysters are shown as examples of (A) individual differences in basal gene expressions (follistatin and intersectin-1); (B) presence or absence (Ø) of basal expression for an interferon-induced 44-like protein (IFI44L) and a baculoviral inhibitor of apoptosis repeat-containing protein 3 homologue (BIRC3). Note that each individual displays a unique profile of gene expression for these four transcripts. Relative expression levels were calculated according to the 2^-ΔΔCq method normalized with the ribosomal L40 protein (Cg-rpl40).