Figure 4

MuMC phylogenomic analysis. Neighbour joining dendrogram based on 128,463 variable common nucleotide positions across 34 sequenced isolates produced by SplitsTree4 [41] using uncorrected ‘P’ distances. The tree was rooted using M. tuberculosis as an outgroup. The major clustering of isolates are M. marinum isolates (4) – blue; Fish and frog isolates (5) – green; Japanese isolate (1) – Mu_8765; French Guiana isolate – Mu_1G897; Australian isolates (10) – orange; African isolates (13) – red. The scale bars show the median pairwise divergence for the set of isolates they span. The isolates that produce mycolactone are highlighted in pale yellow. The inset shows the pMUM plasmid SNP tree of 315 SNPs with edge lengths and a topology matching the corresponding isolates in the main tree. The details of the isolates can be found in Table 1.