Figure 5

RT-PCR showing expression patterns of some of the duplicons in the developmental stages of the parasite. RT-PCR using cDNA libraries of the parasite egg (E), cercaria (C), schistosomula (S) and mixed sex adult (A) as template provide evidence of the transcription and expression of some of the duplicons. The pairs of primers used were designed to amplify the entire coding sequences of the mRNAs. No differential expression pattern was observed but quantitative expression levels were not investigated. (A) Evidence of expression of SjCP1084 protein coding mRNA [GenBank:AY570737], transcribed from the putative source locus [GenBank:CABF01020060]. See Table 2 for list of other similar transcripts. Notably, a non-coding transcript variant [GenBank:FN328299] can also be transcribed from the same locus. See Figure 6 and Additional file 8 for more details. (B) Expression of SjCP3842 protein coding mRNA [GenBank:AY570748] predictably transcribed from [GenBank:CABF01002612]. See Table 2 for list of other similar transcripts in the database. (C) Expression of SjCP1531 [GenBank:AY570742] predictably transcribed from [GenBank:CABF01023364]. See Table 2 for list of other similar transcripts. Notably, a non-coding transcript variant [GenBank:FN329677] can also be transcribed from the same locus (second band). See Figure 6 and Additional file 9 for details. (D) S. japonicum Actin gene was used as internal control to qualify the samples.