Identification of novel MiRNAs and MiRNA expression profiling during grain development in indica rice

BMC Genomics

Table 2 Expression data of miRNAs differentially expressed during the three filling stages

Pattern of regulation	miRNA name	MiRNA expression signals (average)^b			Fold change^b	Fold change^b	p-value^a,c
Pattern of regulation	miRNA name	G1	G2	G3	(G2/G1)	(G3/G2)	p-value^a,c
Up	miR1850	56	53	293	0.95	5.53	7.40E-03
	miR1874-5p	349	1140	1266	3.27	1.11	2.29E-05
	miR1862e	133	378	384	2.84	1.02	3.69E-03
	miR1862d	247	526	656	2.13	1.25	3.17E-03
	miR1874-3p	2473	4684	5507	1.89	1.18	3.57E-03
	miR1435	86	138	357	1.60	2.59	2.24E-03
	miR1881	36	65	88	1.81	1.35	1.78E-03
	*miR1867	109	219	186	2.01	0.85	3.14E-03
	miR1884b	99	213	168	2.15	0.79	3.41E-03
Down	miR171a	118	58	42	0.49	0.72	1.13E-03
	miR171b-f	451	253	182	0.56	0.72	4.64E-03
	miR530-5p	4522	3286	1514	0.73	0.46	4.18E-04
	miR160f	125	38	31	0.30	0.82	1.27E-03
	miR160a-d	251	69	38	0.27	0.55	8.01E-04
	miR160e	275	68	48	0.25	0.71	1.16E-03
	miR444b.2	770	443	63	0.58	0.14	3.68E-04
	*miR1873	123	65	47	0.52	0.72	5.21E-05
	miR2055	138	140	97	1.01	0.69	5.23E-03

*: The probe sequence is on the same precursor of this miRNA, but had a ±2 nt range from the annotated miRNA sequence.
a: p-value < 0.01.
b: Mean levels averaged from three biological replicates; fold changes in miRNA levels were compared from the milky (G1) to hard dough (G3) stages and selected as less than 0.5 or more than 2.0 (from G1 to G3).
c: Signal values <100 (lower than three times the background signal) were not considered for variance analysis.

ISSN: 1471-2164