From: Gene expression and proteomic analysis of the formation of Phakopsora pachyrhizi appressoria
Clone/contig | Target gene product | Primer sequences | Amplicon size (bp) | Primer (nM) | Annealing temperature (°C) | |
---|---|---|---|---|---|---|
Pp30041 | serine/threonine-protein kinase | Forward | GGAACCAACGTCGACAAGAG | 130 | 200 | 60 |
 |  | Reverse | CTGGTCCTCCTCAACCTCAG |  |  |  |
Pp32431 | prefoldin subunit 5 | Forward | AACCTGATCAACTGGCATCC | 81 | 200 | 59 |
 |  | Reverse | CCTTTAGTTGCCCAAACGAG |  |  |  |
Pp32821 | ubiquitin-protein ligase | Forward | AAATTCCTGGAGACGATTGC | 150 | 300 | 60 |
 |  | Reverse | TTGCACTACCTTGTGCGGTA |  |  |  |
Pp34951 | G2/M phase checkpoint control protein Sum2 | Forward | CAGCCTAGAACAGGTCAGATCC | 105 | 200 | 58 |
 |  | Reverse | GCCCGGAAAACGATAAATTC |  |  |  |
Pp35051 | hypothetical protein | Forward | GAGACAAGCCCCATTGAGAG | 104 | 200 | 60 |
 |  | Reverse | GTCTTTGGCAGGGTCTTCTG |  |  |  |
Pp36841 | HMG-CoA reductase | Forward | CGACAGCTTGCTCGAATTATC | 87 | 200 | 60 |
 |  | Reverse | CTTAACCAAGTGTCCAGCTGC |  |  |  |
contig2F1 | 5-aminolevulinate synthase | Forward | GATGAGGTTCACGCCATTG | 117 | 200 | 60 |
 |  | Reverse | GTCCACCCGATCCATTACAC |  |  |  |
Pp31861 | hypothetical protein | Forward | AAGGACTGGTGAGGTTGGTG | 76 | 200 | 62 |
 |  | Reverse | TGCTCTACCACCGTAGGACC |  |  |  |
contig2N1 | P-type cation-transporting ATPase | Forward | GCCTGAAGTGATTCCTCAGC | 124 | 400 | 55 |
 |  | Reverse | ATCCCAACAAACGAAAGTG |  |  |  |
Pp30422 | GTPase-binding protein | Forward | TGCCCAGAAAATTGGTTCTC | 102 | 300 | 58 |
 |  | Reverse | GCCAAAAGTGCATACCGAGT |  |  |  |
Pp32052 | NADPH oxidase A | Forward | ACCCAAGGCTGCCATTAGTA | 134 | 300 | 56 |
 |  | Reverse | GGTGCCCTCCATAAAACAAA |  |  |  |
Pp32222 | septin/cell division control/GTP binding protein | Forward | TCCTCCCAAAGAACATCCAG | 135 | 300 | 60 |
 |  | Reverse | AAGTCTCCATAGCCCGGAGT |  |  |  |
Pp34092 | MFS sugar transporter | Forward | CGGATGTAGCATGGAGACTAATG | 123 | 300 | 60 |
 |  | Reverse | CATCTGAAATCCACCCCTTAGA |  |  |  |
Pp37172 | tripeptidyl-peptidase 1 precursor | Forward | GGCGGAGGGTTTTCAAATTA | 116 | 300 | 59 |
 |  | Reverse | CTTCCTGACCCATCGAACAT |  |  |  |
Pp38882 | microtubule associated protein | Forward | GGGATCAGCTCTACGTCTGC | 150 | 300 | 60 |
 |  | Reverse | AGATTGCCAGCAGCCTTTTA |  |  |  |
Pp39442 | autophagy-related protein 8 | Forward | CCGTATTCCTGTCATCTGTGAG | 107 | 300 | 60 |
 |  | Reverse | CATAGACGAACTGCCCAACC |  |  |  |
Pp39982 | delta (12) fatty acid desaturase | Forward | CCCTCTCCTCCCTCACTACC | 135 | 300 | 59 |
 |  | Reverse | TTGGAGCACAGATGATGAGC |  |  |  |
Pp40002 | subtilase-type proteinase psp3 | Forward | TCACTTTGACATTGGAACGAG | 93 | 300 | 60 |
 |  | Reverse | GCAACCTCAGGAAGGGTTCT |  |  |  |
contig2S2 | acyl-CoA dehydrogenase | Forward | AATCGATACCGCCGTCTATG | 124 | 300 | 60 |
 |  | Reverse | CCTGTGTAAGCCGAAGAAGG |  |  |  |
contig6B2 | conidiation-related protein | Forward | AATGACAGAGGTGGCGAGAC | 107 | 300 | 60 |
 |  | Reverse | TGTCGAAGCTCGTCCTTTTT |  |  |  |
Pp4812 | MAS3 protein | Forward | CGTGATGGTACTCGAACGAAC | 68 | 200 | 62 |
 |  | Reverse | CTTGAAACCTCACGGTCTCG |  |  |  |
NA3 | P. pachyrhizi | Forward | CCAAGGCTTCTTCGTGTTTCA | 65 | 200 | 60 |
 | α-tubulin | Reverse | CAAGAGAAGAGCGCCAAACC |  |  |  |