Developing the anemone Aiptasia as a tractable model for cnidarian-dinoflagellate symbiosis: the transcriptome of aposymbiotic A. pallida

Table 4 Estimating transcriptome completeness by comparison to Nematostella ^a

Pathway	Number of predicted proteins assigned to this pathway in Nematostella	Number of orthologs in Aiptasia transcriptome	Average% of Nematostella coding sequence covered by best alignment	Average predicted amino acid similarity (%)^b
Glycolysis and gluconeogenesis	30	25	89	87
Amino-sugar and nucleotide-sugar metabolism	28	25	68	85
Regulation of autophagy	13	10	63	85
Pentose-phosphate pathway	18	14	81	89
Citrate cycle	28	24	90	89
Valine, leucine, and isoleucine degradation	37	34	80	88
Purine metabolism	92	78	62	86
Fatty acid biosynthesis	5	3	62	79

^aAiptasia orthologs to Nematostella proteins in each pathway (as identified in KEGG) were predicted by best-reciprocal-BLAST analysis (see text).
^b Using BLOSUM62 similarity matrix [41].

ISSN: 1471-2164