Figure 1

Study design. A) Cartoon depicting the allocation of subject samples to flow cells. One high (H) and one low (L) responder was allocated to each flow cell. Within a flow cell, each patient was randomly allocated to lanes 1-4 or 5-8, ensuring that H/L response was balanced over lanes across all flow cells. Finally, for each subject, two technical replicates of their stimulated and unstimulated specimens were randomly allocated to the first two or second two lanes such that stimulation status was balanced over lanes. B) Flow diagram demonstrating the full initial sample set and reasons for excluded lanes of data for the final analysis data set.