Figure 2

Growth (A), acetate production (B) and glucose consumption (C) profiles during batch fermentation (2x SD, 10% glucose). The strains were grown in the presence of 100 (square), 200 (triangle) and 300 (diamond) mM acetoin (NADPH-Bdhp), 200 mM acetoin (NADH-Bdhp, open triangle) and without acetoin (59A, circle). Growth is expressed in function of the amount of CO₂ released determined as described in material and methods. For transcriptome and metabolome analyses, cells were sampled when CO2 reached 6 g/L (indicated by a dotted line). Complete sugar exhaustion was achieved in 36 h for 59A, in 36, 46 and 54 h for NADH-Bdhp in the presence of 100, 200 and 300 mM acetoin, and in 50 h for NADH-Bdhp in the presence of 200 mM acetoin.