Application of next-generation sequencing for rapid marker development in molecular plant breeding: a case study on anthracnose disease resistance in Lupinus angustifolius L.

Table 3 Sequence-specific PCR markers linked to the anthracnose disease resistance Lanr1 of Lupinus angustifolius developed in this study

Marker	Origin	Primer pair	Primer sequences (5’ to 3’)
AnSeq1	Candidate marker 3*	AnSeq1F	AATTCCACAAATTGAAAAAC
		AnSeq1R	GAAGTCAATTAATTAGTATTAGTAC
AnSeq2	Candidate marker 5	AnSeq2F	CTTCTTCAGAAACAAGGAG
		AnSeq2R	CAGATTAATCTCTTTAGATTTTG
AnSeq3	Candidate marker 9	AnSeq3F	GAATTCAGAGACAGGACTC
		AnSeq3R	AGTGTTTTTTTGGTTATCTAG
AnSeq4	Candidate marker 13	AnSeq4F	GAATTCTGATGTGAAACAAC
		AnSeq4R	CTCCTGGCTGAGCTTTG
AnSeq5	Candidate marker 38	AnSeq5F	GAATTCAGTGGAATATTTCAT
		AnSeq5R	CTTAGAACCATTCAGGTATG

*Candidate marker numbers were consistent to the candidate makers listed in Table 1 and Table 2.

ISSN: 1471-2164