Comparative metagenomics of three Dehalococcoides-containing enrichment cultures: the role of the non-dechlorinating community
© Hug et al.; licensee BioMed Central Ltd. 2012
Received: 13 March 2012
Accepted: 1 July 2012
Published: 23 July 2012
The Dehalococcoides are strictly anaerobic bacteria that gain metabolic energy via the oxidation of H2 coupled to the reduction of halogenated organic compounds. Dehalococcoides spp. grow best in mixed microbial consortia, relying on non-dechlorinating members to provide essential nutrients and maintain anaerobic conditions.
A metagenome sequence was generated for the dechlorinating mixed microbial consortium KB-1. A comparative metagenomic study utilizing two additional metagenome sequences for Dehalococcoides-containing dechlorinating microbial consortia was undertaken to identify common features that are provided by the non-dechlorinating community and are potentially essential to Dehalococcoides growth.
The KB-1 metagenome contained eighteen novel homologs to reductive dehalogenase genes. The metagenomes obtained from the three consortia were automatically annotated using the MG-RAST server, from which statistically significant differences in community composition and metabolic profiles were determined. Examination of specific metabolic pathways, including corrinoid synthesis, methionine synthesis, oxygen scavenging, and electron-donor metabolism identified the Firmicutes, methanogenic Archaea, and the ∂-Proteobacteria as key organisms encoding these pathways, and thus potentially producing metabolites required for Dehalococcoides growth.
Comparative metagenomics of the three Dehalococcoides-containing consortia identified that similarities across the three consortia are more apparent at the functional level than at the taxonomic level, indicating the non-dechlorinating organisms’ identities can vary provided they fill the same niche within a consortium. Functional redundancy was identified in each metabolic pathway of interest, with key processes encoded by multiple taxonomic groups. This redundancy likely contributes to the robust growth and dechlorination rates in dechlorinating enrichment cultures.
The Dehalococcoides (Dhc) comprise a genus-level group of bacteria within the phylum Chloroflexi, notable for their ability to respire halogenated compounds including recalcitrant groundwater contaminants[1–4]. Their obligate use of halogenated organic compounds as an energy source has allowed successful development of Dhc-containing enrichment cultures for bioaugmentation of chlorinated ethene-contaminated sites[5, 6]. Within the Dhc group, several strains have been isolated, and there are currently five published genome sequences available[2, 7, 8]. The Dhc genome sequences reveal small, approximately 1.4 Mb chromosomes which encode ~1500 genes and a relatively reduced metabolism[2, 7, 8]. Each Dhc strain contains a unique complement of genes that are homologs of known reductive dehalogenase genes (rdh s), the genes required for respiration of halogenated organic compounds. The characterized reductive dehalogenases are iron-sulfur cluster- and cobalamin-containing membrane-bound components of the Dhc electron transport chain, catalyzing the H2-dependent dehalogenation of a chlorinated substrate with the resultant production of H+ and Cl- ions[9–11]. In a peculiar example of genome streamlining, the Dhc genomes encode only a partial corrinoid synthesis pathway[2, 12], despite a cobalamin cofactor being required for reductive dehalogenase activity and hence Dhc energy production and growth. Isolate cultures of Dhc require exogenous cobalamin amended to the media to allow growth and dechlorination[2, 13–15]. A constraint-based metabolic flux model examining the Dhc core metabolism highlighted the streamlined nature of the Dhc genomes; many typical bacterial pathways, including the TCA cycle and glycolysis are incompletely encoded by this group.
The majority of Dhc-containing cultures are maintained as mixed microbial consortia, primarily because Dhc are notoriously difficult to isolate, but also because mixed cultures exhibit higher Dhc growth rates, higher Dhc titer, and better stability[15–17]. Additionally, mixed cultures are more robust to oxygen exposure, which can kill a pure Dhc culture. The non-dechlorinating members of these cultures are typically fermentative and acetogenic bacteria and methanogens[5, 19–21]. Organic electron donors are fermented to hydrogen and acetate, which Dhc can subsequently utilize. Methanogenic Archaea in the cultures occupy the same niche as dechlorinators, relying on hydrogen and acetate from fermentative and acetogenic bacteria. Collectively, the non-dechlorinating populations help to maintain a reducing environment, and synthesize vitamins and other metabolites that Dhc requires[22–24]. It is hypothesized that the Dhc can scavenge the majority of its required metabolites from the organisms extant in the mixed cultures. This hypothesis was lent weight by experiments in which Dhc pure cultures amended with vitamin B12 (cobalamin) or a B12-producing acetogen showed increased growth and dechlorination rates compared to Dhc grown without an external source of cobalamin. Still, the individual roles of community members in dechlorinating consortia are not well defined. The non-dechlorinating communities coexisting with Dhc show distinct taxonomic compositions in different enrichments; the structure of the community might depend on the electron donor and medium conditions that the cultures are maintained on[21, 25].
The KB-1 consortium is a well-defined enrichment culture originally derived from a TCE-contaminated site in southern Ontario. KB-1 robustly dechlorinates PCE through trichloroethene (TCE), cis-dichloroethene (cis DCE), and vinyl chloride (VC) to ethene, and has been commercialized for use as a bioremediation tool. Previous work with KB-1 has identified the major bacterial and archaeal community members, as well as gene sequences for 14 unique genes homologous to reductive dehalogenases. It contains multiple strains of uncharacterized Dhc and a single Geobacter sp. as active dechlorinating organisms, as well as a variety of methanogens and acetogens.
Physical maintenance conditions for the three Dehalococcoides -containing enrichment cultures
TCE (858 μmol added/L)1
PCE (110 μmol added/L)2
TCE (278 μmol added/L)3
Methanol (4.3 mM)1
Butyrate (440 μM)2
Lactate (25 mM)3
Donor loading rate [(μmol/L/d)/(meeq/L/d)]
Acceptor loading rate [(mmol/L/d)/(meeq/L/d)]
Donor eeq/Acceptor eeq
B12 (0.005 μg/L)
B12 (1 μg/L)
B12 (0.1 μg/L)
KB-1/PCE & KB-1/VC1
ANAS (2 strains) 3
The availability of these metagenomes provides an opportunity to examine the microbial community composition and interactions present in these mixed microbial systems, bypassing the rigorous requirement of isolation of community members and complete genome sequencing. The rapid increase of available metagenome sequences has lead to the development of public servers and software for automated annotation and phylogenetic assignment of metagenome sequences[31–38], facilitating comparative metagenomic studies[39–45]. From metagenomic datasets, community compositions and metabolic functions can be examined. Further functional information in the form of culture-based experiments and/or transcriptomic or proteomic surveys is required to validate the predicted functions. Nevertheless, metagenomic data can suggest functional roles for organisms within a community, and inform follow-on experiments for examining these predicted roles in more detail.
Here we describe the KB-1 enrichment culture metagenome, and include a comparison with the ANAS and DonnaII enrichment consortia based on comparative metagenomics. We conduct an examination of the phylogenetic and metabolic differences and similarities among the three enrichment consortia, with a focus on the non-Dhc microbial population. In addition, we examine the presence and phylogenetic distribution of specific metabolic pathways of interest: cobalamin synthesis, methionine synthesis, oxygen utilization and scavenging, hydrogen production, and metabolism of the electron donors consumed by the enrichment cultures. This in-depth analysis of target pathways identifies taxonomic groups encoding synthesis pathways of required metabolites for Dhc, and strengthens the argument for maintenance of microbial diversity to safeguard functional redundancy within consortia.
Results and discussion
The KB-1 metagenome sequence
General features of the metagenome datasets
Type of sequencing
454 & Sanger
Total number of bases pre-assembly
Number of contigs
Total length of contigs (bp)
Number of singletons
Total length of singletons (bp)
Largest contig (bp)
Average contig size (bp)
Average G + C content (%)
Protein coding genes
- with COGs
- connected to KEGG pathways
rRNA genes (5 S/16 S/23 S)
% Dhc in culture*
Metagenome size (bp)*
Average read length*
Number of sequences*
Number (%) identified for metabolic analysis†
Number (%) identified for phylogenetic analysis†
The KB-1 metagenome was sequenced from three separate clone libraries generated from DNA samples gathered at different dates. The original 3 kb-insert clone library was generated for creation of a shotgun microarray, while the subsequent two DNA samples were gathered specifically for metagenomic sequencing by the JGI, for 3 kb and 35 kb libraries. As a result, the expected community dynamic of the metagenome is a blend of the three DNA samples at the proportions to which they contributed to the final sequence dataset (Figure1A).
A degenerate primer-based clone library of reductive dehalogenase genes generated previously identified 14 unique reductive dehalogenase homologous genes in the KB-1 consortium, named KB1_rdhA1 through A14. Sequencing of the KB-1 metagenome resulted in the identification of a further 18 complete reductive dehalogenase homologous sequences, and 3 partial rdh sequences, bringing the total number of identified reductive dehalogenases in the KB-1 consortium to 35 (Additional file1: Table S1).
Comparative metagenomics of KB-1 with ANAS and DonnaII
The role of the non-dechlorinating community in Dhc- containing enrichment cultures is primarily to ferment the electron donor to H2. The additional processes contributing to the more robust dechlorination in mixed cultures compared to Dhc isolates are not well defined. In order to provide context to an examination of the roles of the KB-1 enrichment culture’s supporting community, we conducted a comparative metagenomic analysis of the three available dechlorinating enrichment culture metagenome sequences. The Joint Genome Institute conducted all three metagenome sequencing projects, under different modes of sequencing (see Table2 for a comparison of metagenome properties).
The assembled datasets and the complete set of raw reads from the three metagenomes were uploaded as separate datasets into the MG-RAST server for automated annotation and phylogenetic assignment. The distribution of different taxonomic groups among the three metagenomes as annotated by MG-RAST is depicted in Figure1B. SEED data on the KB-1 consortium community composition was in general agreement with qPCR and clone library data (Figure1A,) with multiple rare organisms (>1% of the sequence) in the SEED classifications compared to the clone library. We used a hybrid taxonomic classification algorithm, RITA, to provide an independent assessment of the phylogenetic affiliations of the assembled datasets. RITA considers evidence from homology (via nucleotide vs. nucleotide and translated nucleotide vs. protein) and composition, and generated classifications that correlate well with the MG-RAST analysis on the raw read data (Figure1B, Pearson correlation coefficient = 0.930). The main difference between these two sets of phylogenetic assessments is the proportion of Dhc within the datasets, where the annotated raw reads (MG-RAST) provide a more accurate depiction of the enrichment levels of Dhc within these consortia. Due to the small genome size and high enrichment levels, the Dhc contigs have higher read depths, causing the proportion of Dhc sequence in the assembled data to drop. Despite the differences in datasets and classifiers, the two methods largely agree on the taxonomic affiliations of the sequence data.
The MG-RAST analysis included generation of rarefaction curves for the unassembled metagenome sequences, which indicated that none of the three metagenomes have been sequenced to saturation (see MG-RAST metagenome projects for plots and data [MG-RAST IDs 4450840.3 (KB-1 raw reads), 4451142.3 (KB-1 assembled), 4451655.3 (ANAS raw reads), 4478350.3 (ANAS assembled), 4451259.3 (DonnaII raw reads) and 4447020.3 (DonnaII assembled). The distribution and diversity of the taxa identified from the three enrichment cultures indicates that certain organisms have been sequenced at much higher depths (Figure1B). Combined with the absence of close reference genomes for these organisms, it is difficult to estimate the amount of missing information for each organism, meaning that all subsequent analyses are unable to distinguish between missing sequence data and true gene absences within these organisms.
For all subsequent pathway analyses, higher-level taxonomic assignments (phylum or class) were used to describe the presence/absence of pathways in order to reduce the effects of database bias in taxonomic assignments by MG-RAST. It must be noted that in all cases, assignment of a taxonomic identity and putative function to a sequence is dependent entirely on the presence of a known homolog within a sequenced relative in the MG-RAST database. In light of this, identification of novel features in Dhc was not anticipated. Indeed, the focus of this study is on non-Dhc populations, and on their functional gene complements. The requirement for a homolog within the database for gene identification undoubtedly caused a higher proportion of missing information in our analyses, but was an unavoidable constraint that we have attempted to work within.
Broad-scale phylogenetic and metabolic comparison of metagenomes
On a taxonomic level, the STAMP analysis identifies several meaningful differences among the consortia. At the level of phylum, DonnaII exhibits a biological enrichment in Actinobacteria and Synergistetes (Figure3, #6,7), both groups that have been detected in DonnaII, but not in KB-1 or ANAS[1, 19]. ANAS exhibits significant enrichment in Spirochaetes (Figure3, #4). While all three consortia contain methanogenic Archaea at similar proportions (Figure3, #3), the taxonomic affiliations of these methanogens are quite varied. In KB-1, the dominant Archaeal group is order Methanomicrobiales (Figure3, #13), while DonnaII contains families Methanosarcinaceae and Methanosetaceae from order Methanosarcinales (Figure3, #18, 22) and ANAS is enriched in family Methanobacteriaceae from order Metanobacteriales (Figure3, #7). A similar scenario is seen in the Firmicute lineages present in the consortia, with DonnaII enriched in Streptococcaceae and Syntrophomonas (Figure3, #20, 21), ANAS enriched in Clostridiaceae and Caldicellulosiruptor (Figure3, #10, 12) and KB-1 not enriched in any Firmicute genus.
In summary, the broad-scale comparison of the three metagenome sequences identified that the three enrichment consortia metagenomes differ more based on taxonomy (Figure3, Level 5) than they do based on the proportions of the metabolic pathways encoded (Figure4, Level 1). The identified variance in metabolic pathways was confined to either a highly specific function, as in reductive dechlorination by a non-Dhc organism in KB-1, or to specific resistance or mobile element markers, as in the tn552 transposon in DonnaII. Thus at a broad functional level, the communities are remarkably similar. In order to examine whether this similarity was specific to dechlorinating enrichment cultures, or merely the result of a comparison of mixed anaerobic samples, we compared the proportional distribution of genes within COG categories among 25 anaerobic mixed microbial community metagenomes using principal components analysis (Figure5). This analysis also indicated that the three dechlorinating enrichment cultures share a higher level of similarity to each other, on a functional level, than to other, non-bioremediation-related enrichment cultures or anaerobic environmental samples.
Examination of pathways mediating ecological interactions with Dehalococcoides
From information available from published Dehalococcoides genome sequences[2, 7, 8], a metabolic flux model of the group’s metabolism, and culture-based observations, several limitations in Dhc growth and metabolism have been identified. Specifically, the Dhc are incapable of de novo corrinoid synthesis, are obligately anaerobic and highly susceptible to oxygen, and are not able to transform electron donor substrates amended to mixed cultures into the H2 required for energy. In addition, the methionine synthesis pathway in Dhc has not been identified, though Dhc are not methionine auxotrophs. We conducted in-depth examinations of selected metabolic pathways to determine how the mixed consortia may address these deficits and promote Dhc growth. Each pathway of interest was defined through literature searches and the SEED database, and genes pertinent to the processes mined from the MG-RAST annotated metagenomes. Annotations were kept at the phylum or class level to minimize database bias, and gene frequencies were recorded. The numeric data presented in the pathway-specific discussions is primarily from the unassembled datasets, as the assembled datasets corroborated the results from the unassembled data. Complete lists of the specific genes examined for each pathway of interest, as well as the exact gene counts for each phylum within each metagenome for both the unassembled and assembled datasets are available in the Additional file2.
Fermentation of electron donors to hydrogen
Presence of metabolic pathways for the utilization of the electron donor substrates amended to the three enrichment cultures as detected using MG-RAST annotations
# Pathways complete/partial*
Taxonomic Classification of Pathways
A - Lactate to Acetate via Pyruvate (3 genes)
α-P, Firm/Act, BC
Firm/α-P, BC, β-P, Cya, γ-P, Planc
B – Lactate to Ethanol (4 genes)
α-P, Firm/Act, BC
Firm/α-P, BC, β-P, Fib, γ-P
C - L-lactate to Acetate directly (EC 184.108.40.206)
To Acetyl-CoA and Acetoacetate
δ-P, Firm, γ-P, β-P/Act, α-P, BC, Cflx, CrenA, EurA, Fib, Fus
δ-P, Firm/Act, α-P, β-P, EurA, Fus, γ-P
A - to Formaldehyde (ECs 220.127.116.11/18.104.22.168/22.214.171.124)
Act, BC, β-P, DT, δ-P, ϵ-P, EurA, Firm, γ-P
BC, β-P, δ-P, EurA, Firm, γ-P
BC, β-P, δ-P, EurA, Firm, γ-P
B - to Methyl-CoM, eventually to coenzyme M (4 genes)
ANAS is amended lactate as an electron donor, which can be metabolized via pyruvate to acetyl-CoA. From acetyl-CoA, either acetate and hydrogen or ethanol can be formed (KEGG pathway: pyruvate metabolism, Additional file2: Table S3). Another alternative is for lactate to be metabolized through lactaldehyde to propionate. The SEED database does not contain the NADH 1,2-propanediol oxidoreductase required for propionate production. BLAST-based searches of the assembled datasets for this gene indicate it is not present in any of the three metagenomes. This is reasonable, as under low hydrogen partial pressures, and in the presence of methanogens, the energetically favored pathway is via lactate dehydrogenase to pyruvate with acetate as an end product, which is what has been predicted for the ANAS culture. ANAS contains a higher number of taxa encoding this pathway compared to the other two metagenomes (Table3). DonnaII, by comparison, utilizes butyrate as an electron donor, which is ultimately converted to acetyl-CoA and acetoacetate and hydrogen (KEGG pathway: butanoate metabolism, Additional file2: Table S4). The observed trend of organismal diversity exists for butyrate degradation as well, with DonnaII having more hits to distinct phyla associated with this process. KB-1 is provided methanol as an electron donor, which can be transformed to formaldehyde by acetogens or to methyl-coenzyme M en-route to methane formation by methanogens (KEGG pathway: methane metabolism, Additional file2: Table S5). Here, the number of taxonomic groups with complete methanol degradation pathways is highest for DonnaII, with ANAS and KB-1 exhibiting the same number of groups. The electron donor for each enrichment culture was originally chosen based on comparisons of several different donors, which resulted in three different substrates being independently chosen[21, 26, 56]. Interestingly, this analysis predicts that each metagenome still encodes the capacity to utilize any of the electron donors examined here, despite years of exposure to only one of the three substrates. This is valuable information, as a culture’s ability to metabolize multiple electron donors increases its flexibility as a bioremediation tool, though this inferred function would need to be verified prior to implementing a change in electron donor during bioremediation.
The required electron donor for Dhc reductive dechlorination is H2, making it the central intermediate for dechlorination activity in the three consortia. Synthesis of H2 is primarily catalyzed by hydrogenases, a family of enzymes which can reversibly convert hydrogen cations to H2[57, 58]. The three enrichment consortium metagenomes encode a wide diversity of hydrogenases, including nickel-only, nickel-iron, and iron-only hydrogenases, primarily identified in Dhc, the Firmicutes, Euryarchaeota, and the ∂- Proteobacteria (see Additional file1: Table S2 for a more detailed analysis of the hydrogenase family). The identified hydrogenases did not show specific taxonomic trends or enrichment in any of the three metagenomes.
Corrinoid cofactor synthesis
Dhc’s inability to synthesize corrinoid cofactors de novo for reductive dehalogenases required for energy generation means that these cofactors must be scavenged from the environment for Dhc growth[2, 12]. In laboratory cultures, cobalamin is typically amended to the media in the form of vitamin B12 (cyanocobalamin) (see Table1), but in the natural environment, organisms coexisting with Dhc must synthesize it.
An examination of cobalamin synthesis in the non-dechlorinating members of the enrichment cultures yielded no evidence of the aerobic corrinoid synthesis pathway, which involves a later addition of the cobalt cation compared to the anaerobic synthesis pathway. The complete de novo cobalamin synthesis pathway (all 17 genes) was detected in the Euryarchaeota in DonnaII and ANAS, and the Firmicutes in ANAS (Figure6, bottom portion, see Additional file2: Table S6 for more detail). KB-1 showed near-complete representation of the pathway for the Euryarchaeota (13 of 17 genes detected) and both DonnaII and KB-1 exhibited near-completion of the pathway in the Firmicutes (>50% of genes detected, Figure6). The only other phylogenetic group with a significant proportion of the pathway detected was the ∂- Proteobacteria, (14, 15, and 10 of 17 genes detected for DonnaII, ANAS, and KB-1 respectively, Figure6). The incomplete detection of this pathway in the ∂- Proteobacteria is likely the result of sampling effects, as the ∂- Proteobacteria represent a smaller proportion of each of the metagenomes compared to the Firmicutes and Euryarchaeota. Further arguments in favour of a complete corrinoid synthesis pathway within the ∂- Proteobacteria in these enrichment consortia include the uneven distribution of the “absent” genes, which argues against a possible hand-off of a partial product for completion in a different organism; there is no specific break point in the detected pathway in the ∂- Proteobacteria as there is for Dehalococcoides. In addition, in contrast to sequenced Dehalococcoides genomes, which do not encode the upper portion of the corrinoid synthesis pathway, typically complete genomes of ∂- Proteobacteria (e.g., the Geobacteraceae) do encode a complete cobalamin synthesis pathway[60, 61]. It is possible the pathway is indeed incomplete within the ∂- Proteobacteria present in these enrichment cultures, but the available evidence does not favour this hypothesis. The heatmap in Figure6 depicts the extent of the corrinoid synthesis pathway in non-dechlorinating members, either de novo or from various precursor products. Aside from synthesis, import and conversion of corrin-containing molecules functional genes are also primarily found in the Euryarchaeota, the Firmicutes, and the ∂- Proteobacteria.
Presence of methionine biosynthesis pathways in the three enrichment cultures as detected using MG-RAST annotations
# Pathways complete/partial*
Taxonomic Classification of Pathways
Methylation pathway (from methylene-tetrahydrofolate and L-homocysteine)
δ-P, Firm, γ-P
BC, δ-P, Firm
betaine-homocysteine S-methyltransferase (BhmT)
Uptake of Methionine
Methionine transporter MetT
δ-P, Firm, Fuso, γ-P, Spiro
δ-P, Firm, Fuso
ABC Met transporter (3 components)
α-P, β-P, DT, ϵ-P, Firm/Act, γ-P
α-P, ϵ-P, Firm, γ-P/Act, β-P
Based on genome and metagenome sequence data, the enzymes Dhc are utilizing to generate or acquire methionine are divergent from any currently known methionine synthesis enzymes. Identification of the Dhc genes active in methionine synthesis and transport will require targeted experiments examining transcriptomics or protein expression profiles under growth conditions lacking methionine and/or in co-cultures with the Firmicute genera highlighted here as putative methionine producers.
Oxygen tolerance and scavenging
Presence of oxygen-scavenging mechanisms within the three enrichment cultures
Direct O 2 removal
Cytochrome c oxidase
Act, β-P*, Cya*, δ-P*, ϵ-P*, Firm*
Cytochrome d ubiquinol oxidase
Act*, BC, δ-P*, ϵ-P*, Firm
Radical O 2 species
Act, BC, β-P, DT, δ -P, ϵ-P, EurA, Firm, γ-P
BC, β-P, δ-P, EurA, Firm, γ-P
BC, β-P, δ-P, Eury, Firm, γ-P
δ-P, Firm, γ-P
Cytochrome c551 peroxidase
Cya, Firm, Spiro
α-P, β-P, Cya, DT, Dhc, Firm, γ-P, Therm
Dhc, Firm, γ-P, Therm
Dhc, EurA, γ-P
α-P, BC, β-P, Cya, δ-P, γ-P
δ-P, EurA, Firm, Therm
δ-P, EurA, Firm
Putative scavenging systems
BC, Dhc, δ-P, ϵ-P, EurA, Firm
BC, Dhc, δ-P, EurA, Firm
BC, Dhc, δ-P, EurA, Firm
Dhc, ϵ-P, EurA, Firm, γ-P
Dhc, δ-P. EurA, Firm
δ-P, EurA, Firm
δ-P, EurA, Firm
δ-P, EurA, Firm
The ability of the mixed cultures to mitigate oxygen free radical damage is significantly more robust than that of isolate Dhc strains in vivo. In support of this, each metagenome had evidence for at least 2 oxidases, a catalase, peroxidase, multiple kinds of SODs, superoxide reductase, and multiple ruberythrin/rubredoxin scavenging systems in multiple phylogenetic groups (Table5, Additional file2: Table S8). The main organisms encoding these oxygen scavenging systems are the ∂- Proteobacteria, the Firmicutes, and the Euryarchaeota, with Actinobacteria contributing in DonnaII as well. From this, it seems reasonable to conclude that mixed enrichment cultures are more robust to exposure to oxygen because they encode many more enzymes for the complete removal of oxygen and damaging free radical species. Even if only a fraction of these genes are expressed as active proteins, it would still represent a substantial increase in oxygen scavenging mechanisms at work compared to pure Dhc isolate cultures.
Examination of the metabolic and phylogenetic differences among three dechlorinating enrichment cultures revealed that, despite substantial statistically significant differences in phylogenetic groups present, the enrichment cultures show a highly conserved relative abundance of different metabolic pathways. The statistically significant metabolic differences among the three cultures’ non-Dhc populations were restricted to small differences in genes encoding reductive dechlorination and, as seen in many comparative metagenomic studies, the mobile element-associated genes[42, 44, 63]. In addition, a comparison of the metabolic signatures in the metagenomes of a wide variety of anaerobic microbial consortia confirmed that this conserved metabolic profile in the contaminant-degrading consortia is distinct from those found in non-contaminant-degrading anaerobic microbial communities (Figure5).
In particular, the metabolic functions important in supporting Dhc growth were primarily encoded by the Firmicutes, the Euryarchaeota, and the ∂- Proteobacteria, with different genera of each enriched in the three consortia (see Figure2 for an overview of all of the systems examined here). Our results corroborate earlier enrichment culture comparison hypotheses that a diversity of genera can inhabit overlapping functional niches[25, 64].
We postulate that these taxonomic groups are highlighted in the examined pathways because cultures containing them have been best able to support Dhc growth; laboratory enrichment for Dhc activity over time has required the parallel enrichment of these organisms. This analysis identifies a new importance for the Euryarchaeota in these enrichment cultures: while the Euryarchaeota are never the sole predicted source of a metabolite, it is clear from these examinations that they encode pathways predicted to provide essential nutrients, including corrinoids, to the Dhc. The euryarchaeotal contributions to Dhc growth may mitigate or even outweigh their role as H2 competitors with the Dhc, particularly when excess electron donor is present, allowing them to remain abundant in mixed cultures without adverse effects on Dhc growth and dechlorination. The pathway-specific examinations demonstrated that the primary communities maintained in these enrichment consortia metabolize methanol, lactate, or butyrate such that a niche for Dhc growth is created. Notably, each enrichment consortium is maintained on an electron donor chosen for culture performance[21, 26, 56], which has seemingly maintained increased organismal diversity related to donor substrate metabolism in at least two of the three cultures.
In all of the above examinations, it must be reiterated that the identified functions and phylogenetic assignments are predicated on the presence of homologs within the database utilized, and represent predicted functions only. The absence of specific genes on organisms’ genomes cannot be separated from missing data given the unsaturated status of the metagenome sequences. Lateral gene transfers will occlude correct taxonomic identifications. Given the lack of sequence saturation, it is also possible further sequencing of these communities could alter the enrichments identified here. As this analysis relies solely on genomic material, the active function of the pathways examined cannot be confirmed without further proteomic or expression data. Instead, what we have presented here represents a preliminary identification of potential organisms, genes, and pathways of interest within dechlorinating microbial consortia that may serve as guidance for subsequent targeted studies.
Each of these enrichment consortia represents a robust ecosystem allowing an examination of the global halogen cycle, where dechlorination is maintained by the collective activities of the microbial community. The metabolic differences between the enrichment consortia are subtle, which was unexpected given the consortia originated from three disparate environments and have been maintained under different conditions for many years. Taken together, the observations described here illustrate the importance of functional redundancy within dechlorinating enrichment cultures.
Culture and metagenome information
The KB-1 consortium was maintained in batch culture in defined mineral medium with trichloroethene (TCE) as electron acceptor and methanol as electron donor. The culture was routinely allowed to dechlorinate TCE completely to ethene prior to a new amendment of acceptor/donor approximately every two weeks. The DonnaII reactor and ANAS semi-batch reactor were maintained as described previously[19, 20]. See Table1 for a summary comparison of maintenance conditions, and Table2 for metagenome sizes and modes of sequencing used.
For the KB-1 metagenome, DNA was extracted from the T3 MP1 KB-1 culture just after completion of a dechlorination cycle using a Cetyl trimethylammonium bromide (CTAB) protocol with volumes scaled up for higher yield as described in the alternate protocol, omitting subsequent cesium chloride gradient centrifugation steps. Clone libraries with 35-kb and 3-kb inserts were created by the JGI using their in-house protocols (http://www.jgi.doe.gov/sequencing/protocols), and an additional 3 kb short insert library generated for construction of a shotgun metagenome microarray was constructed by Genome Atlantic. A total of 103 MB of metagenome sequence was generated on AB13730xl Sanger sequencers from the three clone libraries. The metagenome was assembled using the JGI’s in-house bacterial assembly pipeline, utilizing lucy for vector and quality screening. The KB-1 metagenome sequence and assembly were made publically available by the JGI on May 2nd, 2009 (http://genome.jgi-psf.org/aqukb/aqukb.download.ftp.html). Community composition of the three KB-1 DNA samples utilized for sequencing the KB-1 metagenome was determined by Dr. Alison S. Waller using qPCR.
The DonnaII metagenome was generated from 454 Titanium libraries according to the JGI’s in-house protocols (http://www.jgi.doe.gov/sequencing/protocols). Metagenome assembly was conducted using the Newbler program from Roche. The sequence data, including an in-house assembly draft for the DonnaII metagenome was made publically available by the JGI on January 31st, 2012 (IMG-M taxon ID: 2032320001 (http://img.jgi.doe.gov/cgi-bin/m/main.cgi)).
The ANAS metagenome was composed of a combination of Sanger sequencing and Titanium 454 sequencing as described above. The sequence data, including an in-house assembly draft for the ANAS metagenome was made publically available by the JGI on August 20th, 2009 (IMG-M taxon ID: 2014730001 (http://img.jgi.doe.gov/cgi-bin/m/main.cgi)).
Comparative metagenome analysis
The raw reads from all three metagenomes as well as the assembled datasets were submitted to the MG-RAST server for automated annotation utilizing the SEED database. All phylogenetic and metabolic profiles discussed herein were generated utilizing a maximum e-value of 1x10-5 and a minimum alignment length match of 100 required, criteria designed to reduce noise and poorly supported assignments, but to still allow imperfect matches between sequences and novel homologs. A global criterion is not ideal for preventing any erroneous annotations, but is required when working with datasets of this size. Vector sequences were identified and removed from the analysis (see Additional file1: materials and methods). In order to facilitate certain comparisons of the non-Dhc communities, all reads assigned to Dhc under MG-RAST’s phylogenetic profiles for the SEED, Silva LSU, Silva SSU, RDP, and Greengenes databases were removed from the datasets, and a second automated annotation conducted for the “Dhc-subtracted” metagenomes.
The assembled datasets were input to the RITA pipeline to generate a second assessment of community composition. RITA combines homology-based predictions with the Naïve Bayes approach to compositional classification used in to generate predictions with increased precision relative to a one-step BLASTN search performed by MG-RAST. The RITA pipeline was used to assign taxonomy to fragments using a reference database of 1479 genomes using the following matching protocols: (i) agreement between the best match with UBLASTX (maximum e-value = 10-15) and the Naïve Bayes classification of a fragment; (ii) a difference of at least ten orders of magnitude between the e-value of the best match and that of the second-best matching group; (iii)-(iv) same as (i)-(ii), but using BLASTN instead of UBLASTX; and (v) matches based on the Naïve Bayes prediction alone. Fragments assigned to set (v) have a much lower confidence than sets (i)-(iv) which are based on homology.
Three-way comparisons were conducted among the metagenomes, adapting the method described by Tringe et al. for MG-RAST output files. In brief, subsystem counts (for metabolic analyses) or taxonomic counts (for a chosen phylogenetic class analysis) were converted to a proportional count for each metagenome. Pseudocounts proportional to the size of each dataset were added to prevent selection for rare categories. For each category, the relative proportions for the three metagenomes were normalized to one. The data were displayed as a barycentric plot, allowing 3-dimensional data to be displayed in 2 dimensions utilizing scripts developed by Tringe et al.. Trends seen in this three-way comparison test were confirmed by the pair-wise statistical testing in STAMP with a p-value of 0.05 (full statistical methods available in the Additional file1: methods), allowing the biological effect size filtering to be indirectly applied to observed three-way differences.
Specific pathway comparisons
For each of the identified pathways or functions of interest, a literature search was conducted to determine all enzymes associated with the system. The SEED database was searched for the subsystem location(s) of the identified genes using enzyme commission (EC) numbers where available, and enzyme names or synonyms as listed in BRENDA (http://www.brenda-enzymes.org) where EC numbers were not available or omitted in the SEED. The complete tabular data for each of subsystems on the resultant list were exported from MG-RAST for each of the three metagenomes.
The SEED phylogenetic identifiers associated with each sequence read were converted from the SEED-specific nomenclature to the NCBI taxonomic identifier, and a phylum-level taxonomic assignment was added. Dhc was designated as a phylum-level classification to distinguish between reads assigned to Dhc and reads assigned to other Chloroflexi. The presence and proportion of the genes of interest across phylogenetic groups were examined (summary tables available in Additional file2).
We would like to thank Mr. Donovan Parks (Dalhousie University) for an introduction to the STAMP program, and Dr. Radhakrishnan Mahadevan (University of Toronto) for critical discussions. Metagenome sequencing for KB-1, DonnaII, and ANAS was conducted by the United States Department of Energy Joint Genome Institute (JGI) community sequencing program. RGB acknowledges the support of Genome Atlantic and the Canada Research Chairs program. LAH was supported by a University of Toronto Fellowship. The authors acknowledge support from the Government of Canada through the Genome Canada and the Ontario Genomics Institute (2009-OGI-ABC-1405) and the United States Department of Defense Strategic Environmental Research and Development Program (SERDP) project ER-1586.
1Department of Cell and Systems Biology, University of Toronto, Toronto, Canada. 2Faculty of Computer Science, Dalhousie University, Halifax, NS, Canada. 3Civil & Environmental Engineering, Cornell University, Ithaca, NY, USA. 4Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada.
- Duhamel M, Mo K, Edwards EA: Characterization of a highly enriched Dehalococcoides-containing culture that grows on vinyl chloride and trichloroethene. Appl Environ Microbiol. 2004, 70: 5538-5545. 10.1128/AEM.70.9.5538-5545.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- Kube M, Beck A, Zinder SH, Kuhl H, Reinhardt R, Adrian L: Genome sequence of the chlorinated compound-respiring bacterium Dehalococcoides species strain CBDB1. Nat Biotechnol. 2005, 23: 1269-1273. 10.1038/nbt1131.View ArticlePubMedGoogle Scholar
- Grostern A, Edwards EA: Growth of Dehalobacter and Dehalococcoides spp. during degradation of chlorinated ethanes. Appl Environ Microbiol. 2006, 72: 428-436. 10.1128/AEM.72.1.428-436.2006.PubMed CentralView ArticlePubMedGoogle Scholar
- Adrian L, Dudkova V, Demnerova K, Bedard DL: "Dehalococcoides" sp. strain CBDB1 extensively dechlorinates the commercial polychlorinated biphenyl mixture aroclor 1260. Appl Environ Microbiol. 2009, 75: 4516-4524. 10.1128/AEM.00102-09.PubMed CentralView ArticlePubMedGoogle Scholar
- Duhamel M, Edwards EA: Growth and yields of dechlorinators, acetogens, and methanogens during reductive dechlorination of chlorinated ethenes and dihaloelimination of 1,2-dichloroethane. Environ Sci Technol. 2007, 41: 2303-2310. 10.1021/es062010r.View ArticlePubMedGoogle Scholar
- Major DW, McMaster ML, Cox EE, Edwards EA, Dworatzek SM, Hendrickson ER, Starr MG, Payne JA, Buonamici LW: Field demonstration of successful bioaugmentation to achieve dechlorination of tetrachloroethene to ethene. Environ Sci Technol. 2002, 36: 5106-5116. 10.1021/es0255711.View ArticlePubMedGoogle Scholar
- Seshadri R, Adrian L, Fouts DE, Eisen JA, Phillippy AM, Methe BA, Ward NL, Nelson WC, Deboy RT, Khouri HM, et al: Genome sequence of the PCE-dechlorinating bacterium Dehalococcoides ethenogenes. Science. 2005, 307: 105-108. 10.1126/science.1102226.View ArticlePubMedGoogle Scholar
- McMurdie PJ, Behrens SF, Muller JA, Goke J, Ritalahti KM, Wagner R, Goltsman E, Lapidus A, Holmes S, Löffler FE, Spormann AM: Localized plasticity in the streamlined genomes of vinyl chloride respiring Dehalococcoides. PLoS Genet. 2009, 5: e1000714-10.1371/journal.pgen.1000714.PubMed CentralView ArticlePubMedGoogle Scholar
- Müller JA, Rösner BM, Von Abendroth G, Meshulam-Simon G, McCarty PL, Spormann AM: Molecular identification of the catabolic vinyl chloride reductase from Dehalococcoides sp. strain VS and its environmental distribution. Appl Environ Microbiol. 2004, 70: 4880-4888. 10.1128/AEM.70.8.4880-4888.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- Maillard J, Schumacher W, Vazquez F, Regeard C, Hagen WR, Holliger C: Characterization of the corrinoid iron-sulfur protein tetrachloroethene reductive dehalogenase of Dehalobacter restrictus. Appl Environ Microbiol. 2003, 69: 4628-4638. 10.1128/AEM.69.8.4628-4638.2003.PubMed CentralView ArticlePubMedGoogle Scholar
- Adrian L, Rahnenfuhrer J, Gobom J, Hölscher T: Identification of a chlorobenzene reductive dehalogenase in Dehalococcoides sp. strain CBDB1. Appl Environ Microbiol. 2007, 73: 7717-7724. 10.1128/AEM.01649-07.PubMed CentralView ArticlePubMedGoogle Scholar
- Ahsanul Islam M, Edwards EA, Mahadevan R: Characterizing the metabolism of Dehalococcoides with a constraint-based model. PLoS Comput Biol. 2010, 6 (8): e1000887-10.1371/journal.pcbi.1000887.PubMed CentralView ArticlePubMedGoogle Scholar
- Lee PK, Cheng D, Hu P, West KA, Dick GJ, Brodie EL, Andersen GL, Zinder SH, He J, Alvarez-Cohen L: Comparative genomics of two newly isolated Dehalococcoides strains and an enrichment using a genus microarray. ISME J. 2011, 5: 1014-1024. 10.1038/ismej.2010.202.PubMed CentralView ArticlePubMedGoogle Scholar
- Maymó-Gatell X, Chien Y, Gossett JM, Zinder SH: Isolation of a bacterium that reductively dechlorinates tetrachloroethene to ethene. Science. 1997, 276: 1568-1571. 10.1126/science.276.5318.1568.View ArticlePubMedGoogle Scholar
- He J, Sung Y, Krajmalnik-Brown R, Ritalahti KM, Löffler FE: Isolation and characterization of Dehalococcoides sp strain FL2, a trichloroethene (TCE)- and 1,2-dichloroethene-respiring anaerobe. Environ Microbiol. 2005, 7: 1442-1450. 10.1111/j.1462-2920.2005.00830.x.View ArticlePubMedGoogle Scholar
- He J, Holmes VF, Lee PK, Alvarez-Cohen L: Influence of vitamin B12 and cocultures on the growth of Dehalococcoides isolates in defined medium. Appl Environ Microbiol. 2007, 73: 2847-2853. 10.1128/AEM.02574-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Cheng D, He J: Isolation and characterization of "Dehalococcoides" sp. strain MB, which dechlorinates tetrachloroethene to trans-1,2-dichloroethene. Appl Environ Microbiol. 2009, 75: 5910-5918. 10.1128/AEM.00767-09.PubMed CentralView ArticlePubMedGoogle Scholar
- Amos BK, Ritalahti KM, Cruz-Garcia C, Padilla-Crespo E, Löffler FE: Oxygen effect on Dehalococcoides viability and biomarker quantification. Environ Sci Technol. 2008, 42: 5718-5726. 10.1021/es703227g.View ArticlePubMedGoogle Scholar
- Richardson RE, Bhupathiraju VK, Song DL, Goulet TA, Alvarez-Cohen L: Phylogenetic characterization of microbial communities that reductively dechlorinate TCE based upon a combination of molecular techniques. Environ Sci Technol. 2002, 36: 2652-2662. 10.1021/es0157797.View ArticlePubMedGoogle Scholar
- Rowe AR, Lazar BJ, Morris RM, Richardson RE: Characterization of the community structure of a dechlorinating mixed culture and comparisons of gene expression in planktonic and biofloc-associated "Dehalococcoides" and Methanospirillum species. Appl Environ Microbiol. 2008, 74: 6709-6719. 10.1128/AEM.00445-08.PubMed CentralView ArticlePubMedGoogle Scholar
- Freeborn RA, West KA, Bhupathiraju VK, Chauhan S, Rahm BG, Richardson RE, Alvarez-Cohen L: Phylogenetic analysis of TCE-dechlorinating consortia enriched on a variety of electron donors. Environ Sci Technol. 2005, 39: 8358-8368. 10.1021/es048003p.View ArticlePubMedGoogle Scholar
- Magnuson JK, Stern RV, Gossett JM, Zinder SH, Burris DR: Reductive dechlorination of tetrachloroethene to ethene by a two-component enzyme pathway. Appl Environ Microbiol. 1998, 64: 1270-1275.PubMed CentralPubMedGoogle Scholar
- Hölscher T, Görisch H, Görisch H, Adrian L: Reductive dehalogenation of chlorobenzene congeners in cell extracts of Dehalococcoides sp. strain CBDB1. Appl Environ Microbiol. 2003, 69: 2999-3001. 10.1128/AEM.69.5.2999-3001.2003.PubMed CentralView ArticlePubMedGoogle Scholar
- Nijenhuis I, Zinder SH: Characterization of hydrogenase and reductive dehalogenase activities of Dehalococcoides ethenogenes strain 195. Appl Environ Microbiol. 2005, 71: 1664-1667. 10.1128/AEM.71.3.1664-1667.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Duhamel M, Edwards EA: Microbial composition of chlorinated ethene-degrading cultures dominated by Dehalococcoides. FEMS Microbiol Ecol. 2006, 58: 538-549. 10.1111/j.1574-6941.2006.00191.x.View ArticlePubMedGoogle Scholar
- Duhamel M, Wehr SD, Yu L, Rizvi H, Seepersad D, Dworatzek S, Cox EE, Edwards EA: Comparison of anaerobic dechlorinating enrichment cultures maintained on tetrachloroethene, trichloroethene, cis-dichloroethene and vinyl chloride. Water Res. 2002, 36: 4193-4202. 10.1016/S0043-1354(02)00151-3.View ArticlePubMedGoogle Scholar
- Waller AS, Krajmalnik-Brown R, Löffler FE, Edwards EA: Multiple reductive-dehalogenase-homologous genes are simultaneously transcribed during dechlorination by Dehalococcoides-containing cultures. Appl Environ Microbiol. 2005, 71: 8257-8264. 10.1128/AEM.71.12.8257-8264.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Holmes VF, He J, Lee PK, Alvarez-Cohen L: Discrimination of multiple Dehalococcoides strains in a trichloroethene enrichment by quantification of their reductive dehalogenase genes. Appl Environ Microbiol. 2006, 72: 5877-5883. 10.1128/AEM.00516-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Rahm BG, Morris RM, Richardson RE: Temporal expression of respiratory genes in an enrichment culture containing Dehalococcoides ethenogenes. Appl Environ Microbiol. 2006, 72: 5486-5491. 10.1128/AEM.00855-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Waller AS: Molecular investigation of chloroethene reductive dehalogenation by the mixed microbial community KB1. 2010, Chemical Engineering and Applied Chemistry: University of TorontoGoogle Scholar
- Parks DH, Beiko RG: Identifying biologically relevant differences between metagenomic communities. Bioinformatics. 2010, 26: 715-721. 10.1093/bioinformatics/btq041.View ArticlePubMedGoogle Scholar
- Meyer F, Paarmann D, D'Souza M, Olson R, Glass EM, Kubal M, Paczian T, Rodriguez A, Stevens R, Wilke A, et al: The metagenomics RAST server - a public resource for the automatic phylogenetic and functional analysis of metagenomes. BMC Bioinforma. 2008, 9: 386-10.1186/1471-2105-9-386.View ArticleGoogle Scholar
- Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA, Formsma K, Gerdes S, Glass EM, Kubal M, et al: The RAST Server: rapid annotations using subsystems technology. BMC Genomics. 2008, 9: 75-10.1186/1471-2164-9-75.PubMed CentralView ArticlePubMedGoogle Scholar
- Overbeek R, Begley T, Butler RM, Choudhuri JV, Chuang HY, Cohoon M, de Crecy-Lagard V, Diaz N, Disz T, Edwards R, et al: The subsystems approach to genome annotation and its use in the project to annotate 1000 genomes. Nucleic Acids Res. 2005, 33: 5691-5702. 10.1093/nar/gki866.PubMed CentralView ArticlePubMedGoogle Scholar
- Huson DH, Auch AF, Qi J, Schuster SC: MEGAN analysis of metagenomic data. Genome Res. 2007, 17: 377-386. 10.1101/gr.5969107.PubMed CentralView ArticlePubMedGoogle Scholar
- Mitra S, Klar B, Huson DH: Visual and statistical comparison of metagenomes. Bioinformatics. 2009, 25: 1849-1855. 10.1093/bioinformatics/btp341.View ArticlePubMedGoogle Scholar
- Seshadri R, Kravitz SA, Smarr L, Gilna P, Frazier M: CAMERA: a community resource for metagenomics. PLoS Biol. 2007, 5: e75-10.1371/journal.pbio.0050075.PubMed CentralView ArticlePubMedGoogle Scholar
- Rodriguez-Brito B, Rohwer F, Edwards RA: An application of statistics to comparative metagenomics. BMC Bioinforma. 2006, 7: 162-10.1186/1471-2105-7-162.View ArticleGoogle Scholar
- Yooseph S, Sutton G, Rusch DB, Halpern AL, Williamson SJ, Remington K, Eisen JA, Heidelberg KB, Manning G, Li W, et al: The Sorcerer II Global Ocean Sampling expedition: expanding the universe of protein families. PLoS Biol. 2007, 5: e16-10.1371/journal.pbio.0050016.PubMed CentralView ArticlePubMedGoogle Scholar
- Sharon I, Battchikova N, Aro EM, Giglione C, Meinnel T, Glaser F, Pinter RY, Breitbart M, Rohwer F, Beja O: Comparative metagenomics of microbial traits within oceanic viral communities. ISME J. 2011, 5: 1178-1190. 10.1038/ismej.2011.2.PubMed CentralView ArticlePubMedGoogle Scholar
- Konstantinidis KT, Braff J, Karl DM, DeLong EF: Comparative metagenomic analysis of a microbial community residing at a depth of 4,000 meters at station ALOHA in the North Pacific subtropical gyre. Appl Environ Microbiol. 2009, 75: 5345-5355. 10.1128/AEM.00473-09.PubMed CentralView ArticlePubMedGoogle Scholar
- Xie W, Wang F, Guo L, Chen Z, Sievert SM, Meng J, Huang G, Li Y, Yan Q, Wu S, et al: Comparative metagenomics of microbial communities inhabiting deep-sea hydrothermal vent chimneys with contrasting chemistries. ISME J. 2011, 5: 414-426. 10.1038/ismej.2010.144.PubMed CentralView ArticlePubMedGoogle Scholar
- Vaishampayan PA, Kuehl JV, Froula JL, Morgan JL, Ochman H, Francino MP: Comparative metagenomics and population dynamics of the gut microbiota in mother and infant. Genome Biol Evol. 2010, 2: 53-66. 10.1093/gbe/evp057.PubMed CentralView ArticlePubMedGoogle Scholar
- Kurokawa K, Itoh T, Kuwahara T, Oshima K, Toh H, Toyoda A, Takami H, Morita H, Sharma VK, Srivastava TP, et al: Comparative metagenomics revealed commonly enriched gene sets in human gut microbiomes. DNA Res. 2007, 14: 169-181. 10.1093/dnares/dsm018.PubMed CentralView ArticlePubMedGoogle Scholar
- Tringe SG, von Mering C, Kobayashi A, Salamov AA, Chen K, Chang HW, Podar M, Short JM, Mathur EJ, Detter JC: Comparative metagenomics of microbial communities. Science. 2005, 308: 554-557. 10.1126/science.1107851.View ArticlePubMedGoogle Scholar
- Waller AS, Hug LA, Mo K, Radford DR, Maxwell KL, Edwards EA: Transcriptional analysis of a Dehalococcoides-containing microbial consortium reveals prophage activation. Appl Environ Microbiol. 2012, 78: 1178-1186. 10.1128/AEM.06416-11.PubMed CentralView ArticlePubMedGoogle Scholar
- McMurdie PJ, Hug LA, Edwards EA, Holmes S, Spormann AM: Site-specific mobilization of vinyl chloride respiration islands by a mechanism common in Dehalococcoides. BMC Genomics. 2011, 12: 287-10.1186/1471-2164-12-287.PubMed CentralView ArticlePubMedGoogle Scholar
- MacDonald NJ, Parks DH, Beiko RG: Rapid identification of high-confidence taxonomic assignments for metagenomic data. Nucleic Acids Res. 2012, 10.1093/nar/gks335Google Scholar
- Parks DH, MacDonald NJ, Beiko RG: Classifying short genomic fragments from novel lineages using composition and homology. BMC Bioinforma. 2011, 12: 328-10.1186/1471-2105-12-328.View ArticleGoogle Scholar
- Livermore DM: beta-Lactamases in laboratory and clinical resistance. Clin Microbiol Rev. 1995, 8: 557-584.PubMed CentralPubMedGoogle Scholar
- Danese I, Haine V, Delrue RM, Tibor A, Lestrate P, Stevaux O, Mertens P, Paquet JY, Godfroid J, De Bolle X, Letesson JJ: The Ton system, an ABC transporter, and a universally conserved GTPase are involved in iron utilization by Brucella melitensis 16 M. Infect Immun. 2004, 72: 5783-5790. 10.1128/IAI.72.10.5783-5790.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- Shultis DD, Purdy MD, Banchs CN, Wiener MC: Outer membrane active transport: structure of the BtuB:TonB complex. Science. 2006, 312: 1396-1399. 10.1126/science.1127694.View ArticlePubMedGoogle Scholar
- Tang YJ, Yi S, Zhuang WQ, Zinder SH, Keasling JD, Alvarez-Cohen L: Investigation of carbon metabolism in "Dehalococcoides ethenogenes" strain 195 by use of isotopomer and transcriptomic analyses. J Bacteriol. 2009, 191: 5224-5231. 10.1128/JB.00085-09.PubMed CentralView ArticlePubMedGoogle Scholar
- Zellner G, Neudorfer F, Diekmann H: Degradation of lactate by an anaerobic mixed culture in a fluidized-bed reactor. Water Res. 1994, 28: 1337-1340. 10.1016/0043-1354(94)90299-2.View ArticleGoogle Scholar
- Men Y, Feil H, VerBerkmoes NC, Shah MB, Johnson DR, Lee PKH, West KA, Zinder SH, Andersen GL, Alvarez-Cohen L: Sustainable syntrophic growth of Dehalococcoides ethenogenes strain 195 with Desulfovibrio vulgaris Hildenborough and Methanobacterium congolense: global transcriptomic and proteomic analyses. International Society for Microbial Ecology. 2012, 2: 410-421.Google Scholar
- Fennell DE, Gossett JM: Modeling the production of and competition for hydrogen in a dechlorinating culture. Environ Sci Technol. 1998, 32: 2450-2460. 10.1021/es980136l.View ArticleGoogle Scholar
- Adams MW, Stiefel EI: Biological hydrogen production: not so elementary. Science. 1998, 282: 1842-1843.View ArticlePubMedGoogle Scholar
- Vignais PM, Billoud B, Meyer J: Classification and phylogeny of hydrogenases. FEMS Microbiol Rev. 2001, 25: 455-501.View ArticlePubMedGoogle Scholar
- Rodionov DA, Vitreschak AG, Mironov AA, Gelfand MS: Comparative genomics of the vitamin B12 metabolism and regulation in prokaryotes. J Biol Chem. 2003, 278: 41148-41159. 10.1074/jbc.M305837200.View ArticlePubMedGoogle Scholar
- Aklujkar M, Krushkal J, DiBartolo G, Lapidus A, Land ML, Lovley DR: The genome sequence of Geobacter metallireducens: features of metabolism, physiology and regulation common and dissimilar to Geobacter sulfurreducens. BMC Microbiol. 2009, 9: 109-10.1186/1471-2180-9-109.PubMed CentralView ArticlePubMedGoogle Scholar
- Methe BA, Nelson KE, Eisen JA, Paulsen IT, Nelson W, Heidelberg JF, Wu D, Wu M, Ward N, Beanan MJ, et al: Genome of Geobacter sulfurreducens: metal reduction in subsurface environments. Science. 2003, 302: 1967-1969. 10.1126/science.1088727.View ArticlePubMedGoogle Scholar
- Zhuang WQ, Yi S, Feng X, Zinder SH, Tang YJ, Alvarez-Cohen L: Selective utilization of exogenous amino acids by Dehalococcoides ethenogenes strain 195 and the effects on growth and dechlorination activity. Appl Environ Microbiol. 2011, 77 (21): 7797-7803. 10.1128/AEM.05676-11.PubMed CentralView ArticlePubMedGoogle Scholar
- Qu A, Brulc JM, Wilson MK, Law BF, Theoret JR, Joens LA, Konkel ME, Angly F, Dinsdale EA, Edwards RA, et al: Comparative metagenomics reveals host specific metavirulomes and horizontal gene transfer elements in the chicken cecum microbiome. PLoS One. 2008, 3: e2945-10.1371/journal.pone.0002945.PubMed CentralView ArticlePubMedGoogle Scholar
- Daprato RC, Löffler FE, Hughes JB: Comparative analysis of three tetrachloroethene to ethene halorespiring consortia suggests functional redundancy. Environ Sci Technol. 2007, 41: 2261-2269. 10.1021/es061544p.View ArticlePubMedGoogle Scholar
- Edwards EA, Grbic-Galic D: Anaerobic degradation of toluene and o-xylene by a methanogenic consortium. Appl Environ Microbiol. 1994, 60: 313-322.PubMed CentralPubMedGoogle Scholar
- Wilson K: Preparation of genomic DNA from bacteria. Curr Protoc Mol Biol. 2001, Chapter 2: Unit 2-4.Google Scholar
- Chou HH, Holmes MH: DNA sequence quality trimming and vector removal. Bioinformatics. 2001, 17: 1093-1104. 10.1093/bioinformatics/17.12.1093.View ArticlePubMedGoogle Scholar
- Margulies M, Egholm M, Altman WE, Attiya S, Bader JS, Bemben LA, Berka J, Braverman MS, Chen YJ, Chen Z, et al: Genome sequencing in microfabricated high-density picolitre reactors. Nature. 2005, 437: 376-380.PubMed CentralPubMedGoogle Scholar
- Edgar RC: Search and clustering orders of magnitude faster than BLAST. Bioinformatics. 2010, 26: 2460-2461. 10.1093/bioinformatics/btq461.View ArticlePubMedGoogle Scholar
- Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol. 1990, 215: 403-410.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.